For immunochemical detection of CD31
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CD31 Rabbit Monoclonal Antibody (Clone RM247)

Item No. 32201

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cluster of Differentiation 31
  • EndoCAM
  • GPIIA
  • PECA1
  • Platelet Endothelial Cell Adhesion Molecule (PECAM-1)
Immunogen
Peptide from the cytoplasmic domain of human CD31
Clone Designation
RM247
MW
~130 kDa
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) CD31
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

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    Cayman Chemical
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    Product Description

    CD31, also known as platelet endothelial cell adhesion molecule (PECAM-1), is a vascular cell adhesion molecule and member of the immunoglobulin (Ig) superfamily of glycoproteins.1 It is a transmembrane glycoprotein that contains a large extracellular domain with Ig homology domains and glycosaminoglycan binding sites, a membrane-spanning region, and a cytoplasmic domain containing multiple phosphorylation sites.1,2 CD31 is expressed on a variety of cells, including endothelial cells, monocytes, neutrophils, and platelets, among others, and is a surface marker for embryonic stem cells (ESCs).1,3 It forms homophilic interactions, which allow it to aggregate at endothelial cell-cell junctions where it regulates vascular permeability.2 Tyrosine phosphorylation at sites on the CD31 cytoplasmic domain, including at immunoreceptor tyrosine-based inhibitory motifs (ITIMs), recruits cytoplasmic proteins, leading to downstream signaling with either inhibitory or activating effects.4 CD31 also has roles in integrin-mediated adhesion, leukocyte recruitment, angiogenesis, and inflammation.2,5,6 High levels of CD31+ circulating cells positively correlate with C-reactive protein (CRP) levels and the Framingham risk score for hard coronary artery disease, while low levels on circulating cells negatively correlate with the Framingham risk score.7 A mutation in PECAM1, the gene encoding CD31, is associated with atherosclerotic cerebral infarction.8 Cayman’s CD31 Rabbit Monoclonal Antibody (Clone RM247) can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes CD31 at approximately 130 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. DeLisser, H.M., Newman, P.J., and Albelda, S.M. Molecular and functional aspects of PECAM-1/CD31. Immunol. Today 15(10), 490-495 (1994).

    2. Lertkiatmongkol, P., Liao, D., Mei, H., et al. Endothelial functions of PECAM-1 (CD31). Curr. Opin. Hematol. 23(3), 253-259 (2016).

    3. Zhao, W., Ji, X., Zhang, F., et al. Embryonic stem cell markers. Molecules 17(6), 6196-6236 (2012).

    4. Newman, P.J., and Newman, D.K. Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arterioscler. Thromb. Vasc. Biol. 23(6), 953-964 (2003).

    5. Newman, P.J. The biology of PECAM-1. J. Clin. Invest. 100(11 Suppl), S25-S29 (1997).

    6. Woodfin, A., Voisin, M.-B., and Nourshargh, S. PECAM-1: A multi-functional molecule in inflammation and vascular biology. Arterioscler. Thromb. Vasc. Biol. 27(12), 2514-2523 (2007).

    7. Ge, Y., Cheng, S., Larson, M.G., et al. Circulating CD31+ leukocyte frequency is associated with cardiovascular risk factors. Atherosclerosis 229(1), 228-233 (2013).

    8. Song, Y., Li, Q., Long, L., et al. Asn563Ser polymorphism of CD31/PECAM-1 is associated with atherosclerotic cerebral infarction in a southern Han population. Neuropsychiatr. Dis. Treat. 11, 15-20 (2014).