For immunochemical detection of α-SMA
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α-SMA (N-Term) Rabbit Monoclonal Antibody (Clone RM253)

Item No. 32204

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • α-Actin 2, Smooth Muscle
  • ACTA2
  • α-Smooth Muscle Actin
Immunogen
Synthetic peptide corresponding to the N-terminus of human α-SMA
Clone Designation
RM253
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody.
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) α-SMA
Species Reactivity
(+) Human(+) Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Product Description

    α-Smooth muscle actin (α-SMA) is a cytoskeletal protein encoded by ACTA2 in humans and a component of the smooth muscle cell (SMC) contractile unit.1 It is expressed in the cytosol of SMCs throughout the vascular system where it polymerizes to form the thin filaments of the SMC contractile unit. α-SMA is also expressed in pericytes and myofibroblasts.2 Acta2 expression is upregulated in, and positively correlates with enhanced migration and contraction of, hepatic stellate cells, which are precursors to myofibroblasts, in a rat model of carbon tetrachloride-induced liver injury. Missense mutations in ACTA2 cause approximately 14% of inherited ascending thoracic aortic aneurysms and dissections (TAAD) via induction of defective SMC contraction, aortic medial degeneration, and SMC hyperplasia or disarray.3 Mutations in ACTA2 are also positively correlated with a predisposition for various additional vascular diseases including premature coronary artery disease, stroke, and Moyamoya disease.4 Cayman’s α-SMA (N-term) Rabbit Monoclonal Antibody (Clone RM253) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Karimi, A., and Milewicz, D.M. Structure of the elastin-contractile units in the thoracic aorta and how genes that cause thoracic aortic aneurysms and dissections disrupt this structure. Can. J. Cardiol. 32(1), 26-34 (2016).

    2. Rockey, D.C., Weymouth, N., and Shi, Z. Smooth muscle α actin (Acta2) and myofibroblast function during hepatic wound healing. PloS One 8(10), e77166 (2013).

    3. Cho, D.-C., Pannu, H., Tran-Fadulu, V., et al. Mutations in smooth muscle alpha-actin (ACTA2) lead to thoracic aortic aneurysms and dissections. Nat. Genet. 39(12), 1448-1493 (2007).

    4. Guo, D.-C., Papke, C.L., Tran-Fadulu, V., et al. Mutations in smooth muscle alpha-actin (ACTA2) cause coronary artery disease, stroke, and Moyamoya disease, along with thoracic aortic disease. Am. J. Hum. Genet. 84(5), 617-627 (2009).