For immunochemical detection of α-cardiac actin
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α-Cardiac Actin (N-Term) Rabbit Monoclonal Antibody (Clone RM257)

Item No. 32211

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • ACTC1
  • Actin
  • alpha Cardiac Muscle 1
Immunogen
Synthetic peptide corresponding to the N-terminal region of human α-cardiac actin
Clone Designation
RM257
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) Human α-cardiac actin(+) Mouse α-cardiac actin
Species Reactivity
(+) Human(+) Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Product Description

    α-Cardiac actin is one of the main actin isoforms found in adult cardiac muscle and the predominant actin in the embryonic heart.1,2 It is a globular protein that forms microfilaments at the Z-disc of sarcomeres and interacts with myosin to perform myofilament, and thus cardiac muscle, contractions.3 Knockout of Actc1, the gene encoding α-cardiac actin, is embryonic lethal in approximately half of mice and generally lethal within two weeks of birth for the other 50%.1 Mutations in ACTC1 are associated with atrial septal defects, familial hypertrophic cardiomyopathy, and idiopathic dilated cardiomyopathy.2,4 Cayman’s α-Cardiac Actin (N-Term) Rabbit Monoclonal Antibody (Clone RM257) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kumar, A., Crawford, K., Close, L., et al. Rescue of cardiac α-actin-deficient mice by enteric smooth muscle γ-actin. Proc. Natl. Acad. Sci. USA 94(9), 4406-4411 (1997).

    2. Matsson, H., Eason, J., Bookwalter, C.S., et al. Alpha-cardiac actin mutations produce atrial septal defects. Hum. Mol. Genet. 17(2), 256-265 (2008).

    3. Sequeira, V., Nijenkamp, L.L.A.M., Regan, J.A., et al. The physiological role of cardiac cytoskeleton and its alterations in heart failure. Biochim. Biophys. Acta 1838(2), 700-722 (2014).

    4. Mogensen, J., Klausen, I.C., Pedersen, A.K., et al. α-cardiac actin is a novel disease gene in familial hypertrophic cardiomyopathy. J. Clin. Invest. 103(10), R39-R43 (1999).