Synaptophysin (C-Term) Rabbit Monoclonal Antibody (Clone RM258)

Synaptophysin is a transmembrane glycoprotein found in synaptic vesicle membranes of the CNS, neuroendocrine tissues, and certain tumors.^1,2 It is comprised of a cytoplasmic N-terminus, four transmembrane domains with the two intravesicular loops containing one disulfide bond per loop, and a cytoplasmic C-terminus containing ten pentapeptide repeat domains.¹ Nine of the repeat domains begin with a tyrosine residue subject to phosphorylation.³ Synaptophysin interacts with the v-SNARE synaptobrevin and the GTPase dynamin to facilitate exo-endocytosis coupling in the release of neurotransmitters.^3,4,5 Mutations in SYP, the gene encoding synaptophysin, are associated with X-linked intellectual disability.⁶ Synaptophysin has commonly been used as a marker of synapses and synapse vesicles in the brain.¹ Cayman’s Synaptophysin (C-Term) Rabbit Monoclonal Antibody (Clone RM258) can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes synaptophysin at approximately 38 kDa from human samples.

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1. Valtorta, F., Pennuto, M., Bonanomi, D., et al. Synaptophysin: leading actor or walk-on role in synaptic vesicle exocytosis? Bioessays 26(4), 445-453 (2004).

2. Wiedenmann, B., Franke, W.W., Kuhn, C., et al. Synaptophysin: a marker protein for neuroendocrine cells and neoplasms. Proc. Natl. Acad. Sci. USA 83(10), 3500-3504 (1986).

3. Evans, G.J.O., and Cousin, M.A. Tyrosine phosphorylation of synaptophysin in synaptic vesicle recycling. Biochem. Soc. Trans. 33(Pt 6), 1350-1353 (2005).

4. Kwon, S.E., and Chapman, E.R. Synaptophysin regulates the kinetics of synaptic vesicle endocytosis in central neurons. Neuron 70(5), 847-854 (2011).

5. Xie, Z., Long, J., Liu, J., et al. Molecular mechanisms for the coupling of endocytosis to exocytosis in neurons. Front. Mol. Neurosci. 10, 47 (2017).

6. Tarpey, P.S., Smith, R., Pleasance, E., et al. A systematic, large-scale resequencing screen of X-chromosome coding exons in mental retardation. Nat. Genet. 41(5), 535-543 (2009).