For immunochemical detection of cytokeratin 8
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Cytokeratin 8 (C-Term) Rabbit Monoclonal Antibody (Clone RM266)

Item No. 32216

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Keratin, Type II Cytoskeletal 8
  • CK-8
  • Keratin-8
  • KRT8
Immunogen
Peptide from the C-terminal region of human cytokeratin 8
Clone Designation
RM266
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC and WB
Cross Reactivity
(+) Cytokeratin 8
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Cayman Chemical
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    Product Description

    Cytokeratin 8 is a type II epithelial intermediate filament protein.1 It is composed of a central rod containing four α-helical domains, which are important for self-assembly, and non-helical head and tail domains at the N- and C-termini, respectively. Cytokeratin 8 is expressed in single-layered epithelial cells and epithelial-derived tumor cells.2 It dimerizes with the type I epithelial intermediate filament protein cytokeratin 18 to form a network of filament bundles throughout the cytoplasm.3 The tail of cytokeratin 8 binds to plasminogen and mediates invasiveness of cancer cells in vitro.4 A null mutation in KRT8, the gene encoding cytokeratin 8, is embryonic lethal in C57BL/6 x 129Sv, but not FVB/N, mice, which develop colorectal hyperplasia.5 Substitution mutations in KRT8, corresponding to residues in the head domain of the protein, disrupt filament reorganization following oxidative and non-oxidative stress in vitro and are associated with cryptogenic liver disease.2 Intratumoral levels of cytokeratin 8 and -18 are correlated with poor prognosis in patients with squamous cell carcinomas of the oral cavity.6 Cayman’s Cytokeratin 8 (C-Term) Rabbit Monoclonal Antibody (Clone RM266) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gu, L.-H., and Coulombe, P.A. Keratin function in skin epithelia: A broadening palette with surprising shades. Curr. Opin. Cell Biol. 19(1), 13-23 (2007).

    2. Ku, N.-O., Gish, R., Wright, T.L., et al. Keratin 8 mutations in patients with cryptogenic liver disease. N. Engl. J. Med. 344(21), 1580-1587 (2001).

    3. Gonias, S.L., Hembrough, T.A., and Sankovic, M. Cytokeratin 8 functions as a major plasminogen receptor in select epithelial and carcinoma cells. Front Biosci. 6, 1403-1411 (2001).

    4. Obermajer, N., Doljak, B., and Kos, J. Cytokeratin 8 ectoplasmic domain binds urokinase-type plasminogen activator to breast tumor cells and modulates their adhesion, growth and invasiveness. Mol. Cancer 8, 88 (2009).

    5. Baribault, H., Penner, J., Iozzo, R.V., et al. Colorectal hyperplasia and inflammation in keratin 8-deficient FVB/N mice. Genes Dev. 8(24), 2964-2973 (1994).

    6. Fillies, T., Werkmeister, R., Packeisen, J., et al. Cytokeratin 8/18 expression indicates a poor prognosis in squamous cell carcinomas of the oral cavity. BMC Cancer 6, 10 (2006).