For immunochemical detection of Smac
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Smac (C-Term) Rabbit Monoclonal Antibody (Clone RM271)

Item No. 32221

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • DIABLO
  • Diablo Homolog, Mitochondrial
  • Direct IAP-binding Protein with Low pI
  • Second Mitochondria-derived Activator of Caspase
Immunogen
Peptide from the C-terminal region of human Smac
Clone Designation
RM271
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC and WB
Cross Reactivity
(+) Smac
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    Smac is a mitochondrial protein involved in the initiation of apoptosis.1 It is a ubiquitously expressed α-helical protein that forms homodimers.1,2 It is localized to the mitochondria but is released following apoptotic stimuli into the cytoplasm, where it binds to inhibitor of apoptosis proteins (IAPs), which are overexpressed in a variety of cancer cells.1,3 Smac binding to IAPs blocks their inhibitory effect on caspase-3, -7, and -9 and leads to the activation of apoptosis.4,3 Knockout of DIABLO, the gene encoding Smac, in colon cancer cells in vitro induces resistance to apoptosis induced by NSAIDs and TRAIL, but not cisplatin (Item No. 13119), mitomycin C (Item No. 11435), or camptothecin (Item No. 11694).5 DIABLO knockout in mice does not induce changes in apoptosis or tumorigenesis, indicating a redundancy of its function.3 Overexpression of DIABLO in vitro does not affect basal levels of apoptosis but increases the sensitivity of cells to other apoptotic stimuli.6 Cayman’s Smac Rabbit Monoclonal Antibody (Clone RM271) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Du, C., Fang, M., Li, Y., et al. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell 102(1), 33-42 (2000).

    2. Wu, G., Chai, J., Suber, T.L., et al. Structural basis of IAP recognition by Smac/DIABLO. Nature 408(6815), 1008-1012 (2000).

    3. Abbas, R., and Larisch, S. Targeting XIAP for promoting cancer cell death-the story of ARTS and SMAC. Cells 9(3), 663 (2020).

    4. Shi, Y. A structural view of mitochondria-mediated apoptosis. Nat. Struct. Biol. 8(5), 394-401 (2001).

    5. Kohli, M., Yu, J., Seaman, C., et al. SMAC/Diablo-dependent apoptosis induced by nonsteroidal antiinflammatory drugs (NSAIDs) in colon cancer cells. Proc. Natl. Acad. Sci. USA 101(48), 16897-16902 (2004).

    6. Guo, F., Nimmanapalli, R., Paranawithana, S., et al. Ectopic overexpression of second mitochondria-derived activator of caspases (Smac/DIABLO) or cotreatment with N-terminus of Smac/DIABLO peptide potentiates epothilone B derivative-(BMS 247550) and Apo-2L/TRAIL-induced apoptosis. Blood 99(9), 3419-3426 (2002).