JAM-A (N-Term) Rabbit Monoclonal Antibody (Clone RM275)

Junctional adhesion molecule A (JAM-A), known previously as JAM-1, is a type I transmembrane protein and member of the immunoglobulin (Ig) superfamily that regulates epithelial and endothelial barrier and tight junction formation.^1,2,3 It is comprised of an N-terminal extracellular domain containing two Ig-like domains that mediate cell-cell interactions, a single transmembrane domain, and a cytoplasmic tail containing a PDZ binding motif that interacts with numerous cytoplasmic scaffold proteins and intracellular signaling molecules.^1,2 JAM-A exists as a monomer or homodimer, is primarily expressed by epithelial cells, endothelial cells, and leukocytes, and localizes to the cell surface.¹ It associates via cis- or trans-interactions with a variety of cell surface proteins, including the additional JAM family members JAM-B and JAM-C, which are also expressed by epithelial cells, endothelial cells, and leukocytes, as well as integrins expressed by leukocytes, such as αLβ2, αMβ2, and α4β1, to mediate homotypic or heterotypic cell-cell adhesions that regulate tight junction assembly and transepithelial cell migration. JAM-A has additional roles in angiogenesis, hemostasis, cell cycle, and inflammation. JAM-A levels are increased in plasma cells isolated from patients with multiple myeloma and are associated with poor prognosis.⁴ Cayman's JAM-A (N-Term) Rabbit Monoclonal Antibody (Clone RM275) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Ebnet, K. Junctional adhesion molecules (JAMs): Cell adhesion receptors with pleiotropic functions in cell physiology and development. Physiol. Rev. 97(4), 1529-1554 (2017).

2. Mandell, K.J., Babbin, B.A., Nusrat, A., et al. Junctional adhesion molecule 1 regulates epithelial cell morphology through effects on β1 integrins and Rap1 activity. The Journal of Biological Chemisty 280(12), 11665-11674 (2005).

3. Mandell, K.J., McCall, I.C., and Parkos, C.A. Involvement of the junctional adhesion molecule-1 (JAM1) homodimer interface in regulation of epithelial barrier function. The Journal of Biological Chemisty 279(16), 16254-16262 (2004).

4. Solimando, A.G., Brandl, A., Mattenheimer, K., et al. JAM-A as a prognostic factor and new therapeutic target in multiple myeloma. Leukemia 32(3), 736-743 (2018).