For immunochemical detection of cytokeratin 20
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Cytokeratin 20 (C-Term) Rabbit Monoclonal Antibody (Clone RM283)

Item No. 32232

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CK-20
  • Keratin-20
  • Keratin, Type II Cytoskeletal 20
  • KRT20
Immunogen
Peptide from the C-terminal region of human cytokeratin 20
Clone Designation
RM283
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody.
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) Cytokeratin 20
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    Cytokeratin 20 (CK-20) is an intermediate filament protein and a member of the cytokeratin protein family.1,2 It is a type I acidic cytokeratin expressed in intestinal epithelium, gastric foveolar epithelium, urothelium, and Merkel cells and is localized in the cytoplasm.3 CK-20 is found in various tumors of mucosal origin, including adenocarcinomas of the large and small intestine, ovarian cancers, and Merkel cell carcinomas.4 It is also found in urothelial carcinomas and in adenocarcinomas of the bile ducts, pancreas, and stomach, but is not present in primary epithelial tumors of non-mucosal origin, and has been used as a biomarker of tumor origin.4,5 Cayman’s Cytokeratin 20 (C-Term) Rabbit Monoclonal Antibody (Clone RM283) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Moll, R., Schiller, D.L., and Franke, W.W. Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J. Cell Biol. 111(2), 567-580 (1990).

    2. Weng, Y.-R., Cui, Y., and Fang, J.-Y. Biological functions of cytokeratin 18 in cancer. Mol. Cancer Res. 10(4), 485-493 (2012).

    3. Moll, R., Löwe, A., Laufer, J., et al. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am. J. Pathol. 140(2), 427-447 (1992).

    4. Rabban, J.T., Soslow, R.A., and Zaloudek, C.Z. Immunohistology of the female genital tract. Diagnostic immunohistochemistry 690-762 (2010).

    5. Hernandez, B.Y., Frierson, H.F., Moskaluk, C.A., et al. CK20 and CK7 protein expression in colorectal cancer: Demonstration of the utility of a population-based tissue microarray. Hum. Pathol. 36(3), 275-281 (2005).