α-Actinin-4 (N-Term) Rabbit Monoclonal Antibody (Clone RM287)

α-Actinin-4 (ACTN4) is a filamentous actin-crosslinking protein and member of the α-actinin protein family with roles in the regulation of cytoskeletal integrity and cell movement, as well as gene transcription.^1,2 It is composed of an N-terminal actin-binding region with two calponin-homology repeats, a rod domain containing four spectrin-like repeats that is essential for anti-parallel dimerization, and a C-terminal calmodulin-like domain.¹ ACTN4 is ubiquitously expressed in non-muscle cells and primarily localized to the cytoplasm where it participates in cytoskeletal organization.² However, ACTN4 can be translocated to the nucleus and induce transcription via interaction with nuclear receptors, including estrogen receptor α, retinoic acid receptors (RARs), and the vitamin D receptor, and acts as a transcriptional co-activator of NF-κB. Mutation of ACTN4 induces podocyte injury caused by cytoskeletal dysregulation and proteotoxicity and has been seen in patients with hereditary focal segmental glomerulosclerosis.³ ACTN4 is overexpressed and associated with tumor metastasis in various cancers.² Cayman's α-Actinin-4 (N-Term) Rabbit Monoclonal Antibody (Clone RM287) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

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1. Zhang, Y.Y., Tabataba, H., Liu, X.Y., et al. ACTN4 regulates the stability of RIPK1 in melanoma. Oncogene 37(29), 4033-4045 (2018).

2. Jung, J., Kim, S., An, H.-T., et al. α-Actinin-4 regulates cancer stem cell properties and chemoresistance in cervical cancer. Carcinogenesis 41(7), 940-949 (2020).

3. Yee, A., Papillon, J., Guillemette, J., et al. Proteostasis as a therapeutic target in glomerular injury associated with mutant α-actinin-4. Am. J. Physiol. Renal Physiol. 315(4), F954-F966 (2018).