For immunochemical detection of eIF2α (phospho-Ser51)
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eIF2α (Phospho-Ser51) Rabbit Monoclonal Antibody (Clone RM298)

Item No. 32244

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Eukaryotic Translation Initiation Factor 2 Subunit 1
Immunogen
Phosphopeptide corresponding to human eIF2α (phospho-Ser51)
Clone Designation
RM298
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) eIF2α(-) eIF2α without phosphorylation at serine 51
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Cayman Chemical
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    Product Description

    eIF2α is the α subunit of eukaryotic inhibition factor 2, which also includes eIF2β and eIF2γ subunits and is involved in the initiation of translation.1 eIF2α is composed of a globular N-terminal domain, which contains an S1 domain and an α-helical domain, and a globular C-terminal domain.2 eIF2α can be phosphorylated at serine 51 by the eIF2α kinases HRI/EIF2AK1, PKR/EIF2AK2, PERK/EIF2AK3, and GCN2/EIF2AK4 following various cell and ER stressors, viral invasion, or nutrient deprivation, among other factors.3,4,5 Phosphorylation of eIF2α at serine 51 promotes the interaction between eIF2α and the guanine nucleotide exchange factor eIF2B, which inhibits eIF2B activity and prevents the initiation of translation, reducing global protein synthesis in the cell.1 The transcription of specific factors involved in the integrated stress response (ISR), such as ATF4, is upregulated by eIF2α phosphorylation.3 Dephosphorylation of eIF2α by the protein phosphatase 1 (PP1) complex, which includes GADD34 and CReP, terminates the ISR and returns protein synthesis back to basal levels. Cayman’s eIF2α (Phospho-Ser51) Rabbit Monoclonal Antibody (Clone RM298) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sudhakar, A., Ramachandran, A., Ghosh, S., et al. Phosphorylation of serine 51 in initiation factor 2α (eIF2α) promotes complex formation between eIF2α(P) and eIF2B and causes inhibition in the guanine nucleotide exchange activity of eIF2B. Biochemistry 39(42), 12929-12938 (2000).

    2. Ito, T., Marintchev, A., and Wagner, G. Solution structure of human initiation factor eIF2α reveals homology to the elongation factor eEF1B. Structure 12(9), 1693-1704 (2004).

    3. Pakos-Zebrucka, K., Koryga, I., Mnich, K., et al. The integrated stress response. EMBO Rep. 17(10), 1374-1395 (2016).

    4. Humeau, J., Leduc, M., Cerrato, G., et al. Phosphorylation of eukaryotic initiation factor-2α (eIF2α) in autophagy. Cell Death Dis. 11(6), 433 (2020).

    5. Muaddi, H., Majumder, M., Peidis, P., et al. Phosphorylation of eIF2α at serine 51 is an important determinant of cell survival and adaptation to glucose deficiency. Mol. Biol. Cell 21(18), 3220-3231 (2010).