For immunochemical detection of p27Kip1
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p27Kip1 (C-Term) Rabbit Monoclonal Antibody (Clone RM302)

Item No. 32247

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CDKN4
  • Cyclin-dependent Kinase Inhibitor P27
  • Cyclin-dependent Kinase Inhibitor 1B
  • KIP1
Immunogen
Peptide from the C-terminal region of human p27Kip1
Clone Designation
RM302
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
50% glycerol/PBS, with 1% BSA and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) p27Kip1
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    p27Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase (CDK) inhibitor proteins encoded by CDKN1B in humans.1 It is an intrinsically disordered protein but does contain a structured N-terminal kinase inhibitor domain composed of a nuclear export signal, cyclin-binding subdomain (D1), CDK-binding subdomain (D2) that binds to CDKs to inhibit their catalytic activity, and linker subdomain that connects D1 and D2. p27Kip1 is ubiquitously expressed and shuttles from the nucleus to the cytoplasm in a cell cycle- or signaling-dependent manner. It has subcellular compartment-specific roles in cytoskeletal organization, autophagy, cytokinesis, and regulation of transcription, as well as regulation of cell proliferation, survival, differentiation, invasion, and motility.2 Germline mutations in CDKN1B have been found in tumor samples isolated from patients with multiple endocrine neoplasia type 1 and familial prostate cancer. Epithelial and stromal levels of p27Kip1 are decreased in women with endometriosis.3 Cayman’s p27Kip1 (C-Term) Rabbit Monoclonal Antibody (Clone RM302) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bachs, O., Gallastegui, E., Orlando, S., et al. Role of p27Kip1 as a transcriptional regulator. Oncotarget 9(40), 26259-26278 (2018).

    2. Cusan, M., Mungo, G., Zompit, M.D.M., et al. Landscape of CDKN1B mutations in luminal breast cancer and other hormone-driven human tumors. Front. Endocrinol. (Lausanne) 9, 393 (2018).

    3. Gonçalves, G.A. p27kip1 as a key regulator of endometriosis. Eur. J. Obstet. Gynecol. Reprod. Biol. 221, 1-4 (2018).