For immunochemical detection of PLAP
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

PLAP (N-Term) Rabbit Monoclonal Antibody (Clone RM317)

Item No. 32259

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Peptide from the N-terminal region of human PLAP
Clone Designation
RM317
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC and WB
Cross Reactivity
(+) PLAP
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Product Description

    Placental alkaline phosphatase (PLAP) is the placenta-specific isoenzyme of AP encoded by the ALPP gene in humans.1,2 It exists as a homodimer where each monomer is composed of an N-terminal α-helix and a crown domain that stabilize PLAP homodimers, a Zn2+- and Mg2+-containing active site with a catalytic serine residue, and a non-catalytic metal-binding site occupied by Ca2+.3,4 PLAP is predominately expressed by syncytiotrophoblasts in the placenta and is tethered to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor.4,3 It is also present at low levels in the cervix, ovaries, and type I pneumocytes, as well as in the serum.1,3 Increased PLAP activity has been found in tumor tissue and cerebrospinal fluid (CSF) isolated from patients with intracranial germ cell tumors.5 Cayman's PLAP (N-Term) Rabbit Monoclonal Antibody (Clone RM317) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zaher, D.M., El-Gamal, M.I., Omar, H.A., et al. Recent advances with alkaline phosphatase isoenzymes and their inhibitors. Arch. Pharm. (Weinheim) 353(5), e2000011 (2020).

    2. Wennberg, C., Kozlenkov, A., Di Mauro, S., et al. Structure, genomic DNA typing, and kinetic characterization of the D allozyme of placental alkaline phosphatase (PLAP/ALPP). Hum. Mutat. 19(3), 258-267 (2002).

    3. Millán, J.L. Alkaline phosphatases : Structure, substrate specificity and functional relatedness to other members of a large superfamily of enzymes. Purinergic Signal. 2(2), 335-341 (2006).

    4. Le Du, M.H., Stigbrand, T., Taussig, M.J., et al. Crystal structure of alkaline phosphatase from human placenta at 1.8 Å resolution. Implication for a substrate specificity. The Journal of Biological Chemisty 276(12), 9158-9165 (2001).

    5. Aihara, Y., Watanabe, S., Amano, K., et al. Placental alkaline phosphatase levels in cerebrospinal fluid can have a decisive role in the differential diagnosis of intracranial germ cell tumors. J. Neurosurg. 131(3), 687-694 (2018).