For immunochemical detection of BAG-1L
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BAG-1L Rabbit Monoclonal Antibody (Clone RM310)

Item No. 32261

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • BAG Co-chaperone 1L
  • BAG Family Molecular Chaperone Regulator 1L
  • Bcl-2-associated Anthanogene-1L
Immunogen
Peptide corresponding to a region near the N-terminus of human BAG-1L
Clone Designation
RM310
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody.
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ICC, IHC, WB
Cross Reactivity
(+) BAG-1L(-) BAG-1M(-) BAG-1S
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $476

    Add
    Cayman Chemical
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    Product Description

    Bcl-2-associated anthanogene 1 (BAG-1) is a multifunctional co-chaperone protein with roles in apoptosis and transcription, as well as cell motility, survival, and proliferation.1,2 It is encoded by a single gene, BAG1, that produces several isoforms with variable N-termini through alternate translation initiation of a single mRNA transcript.3 BAG-1L, the longest isoform, is localized to the nucleus and composed of a nuclear localization sequence, a basic DNA-binding domain, a ubiquitin-like domain, and an Hsp70- and Hsc70-binding domain.2 It functions as a co-chaperone to enhance transactivation of the androgen receptor via interaction with the partially folded and intrinsically disordered N-terminal tau-5 domain, which stabilizes the receptor to facilitate chromatin binding and activates gene transcription.4 Overexpression of BAG-1L inhibits apoptosis induced by cisplatin (Item No. 13119) in HeLa cells stably transfected with pEZ-M02-BAG-1L plasmid DNA, and BAG-1L levels are increased in tumors isolated from patients with castration-resistant prostate cancer (CRPC).3,4 Cayman’s BAG-1L Rabbit Monoclonal Antibody (Clone RM310) can be used for immunocytochemistry (ICC), immunohistochemistry (ICC), and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dobbyn, H.C., Hill, K., Hamilton, T.L., et al. Regulation of BAG-1 IRES-mediated translation following chemotoxic stress. Oncogene 27(8), 1167-1174 (2008).

    2. Gehring, U. Activities of the cochaperones Hap46/BAG-1M and Hap50/BAG-1L and isoforms. Cell Stress Chaperones 11(4), 295-303 (2006).

    3. Ozfiliz, P., Arisan, E.D., Coker-Gurkan, A., et al. Bag-1L is a stress-withstand molecule prevents the downregulation of Mcl-1 and c-Raf under control of heat shock proteins in cisplatin treated HeLa cervix cancer cells. Asian Pac. J. Cancer Prev. 15(11), 4475-4482 (2014).

    4. Cato, L., Neeb, A., Sharp, A., et al. Development of Bag-1L as a therapeutic target in androgen receptor-dependent prostate cancer. Elife 6, e27159 (2017).