Cytokeratin 14 (C-Term) Rabbit Monoclonal Antibody (Clone RM328)

Cytokeratin 14 (CK-14) is a type I acidic epithelial intermediate filament protein.¹ It is composed of a central rod containing four α-helical domains, which are important for self-assembly, and non-helical head and tail domains at the N- and C-termini, respectively.^2,3 CK-14 is expressed in stratified squamous epithelial cells, keratinocytes, and myoepithelial cells in the basal layer of the epidermis and is an integral component of the epithelial cell cytoskeleton.^1,4,5 It dimerizes with the type II basic epithelial intermediate filament protein CK-5 via heptad repeats in the central rod domain to form a network of filament bundles throughout the cytoplasm.^2,3 Increased tumor levels of CK-14 have been found in patients with squamous cell carcinomas of the bladder, lung, or cervix.⁶ Mutations in the gene encoding CK-14 have been found in patients with epidermolysis bullosa simplex (EBS), a disorder characterized by skin blistering.⁷ Cayman's Cytokeratin 14 (C-Term) Rabbit Monoclonal Antibody (Clone RM328) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

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1. Asano-Miyoshi, M., Hamamichi, R., and Emori, Y. Cytokeratin 14 is expressed in immature cells in rat taste buds. J. Mol. Histol. 39(2), 193-199 (2008).

2. Bousquet, O., Ma, L., Yamada, S., et al. The nonhelical tail domain of keratin 14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro. J. Cell. Biol. 155(5), 747-754 (2001).

3. Lee, C.-H., Kim, M.-S., Chung, B.M., et al. Structural basis for heteromeric assembly and perinuclear organization of keratin filaments. Nat. Struct. Mol. Biol. 19(7), 707-715 (2012).

4. Törmä, H. Regulation of keratin expression by retinoids. Dermatoendocrinol. 3(3), 136-140 (2011).

5. Reis-Filho, J.S., Simpson, P.T., Martins, A., et al. Distribution of p63, cytokeratins 5/6 and cytokeratin 14 in 51 normal and 400 neoplastic human tissue samples using TARP-4 multi-tumor tissue microarray. Virchows Arch. 443(2), 122-132 (2003).

6. Chu, P.G., Lyda, M.H., and Weiss, L.M. Cytokeratin 14 expression in epithelial neoplasms: A survey of 435 cases with emphasis on its value in differentiating squamous cell carcinomas from other epithelial tumours. Histopathology 39(1), 9-16 (2001).

7. Atkinson, S.D., McGilligan, V.E., Liao, H., et al. Development of allele-specific therapeutic siRNA for keratin 5 mutations in epidermolysis bullosa simplex. J. Invest. Dermatol. 131(10), 2079-2086 (2011).