For immunochemical detection of MART1
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MART1 (N-Term) Rabbit Monoclonal Antibody (Clone RM333)

Item No. 32270

Product Insert (PDF)
Technical Information
Synonyms
  • Epididymis Secretory Sperm Binding Protein
  • Melan-A
  • Melanoma Antigen Recognized by T cells 1
  • MLANA
Immunogen
Peptide from the N-terminal region of human MART1
Clone Designation
RM333
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) MART1
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    MART1 is a type III membrane protein encoded by MLANA in humans.1,2 It is expressed in melanocytes and localized to melanosomes, the endoplasmic reticulum, and in the trans-Golgi network. It is also expressed in retinal pigment epithelium and melanoma cells.3 MART1 antigenic peptides are expressed in an MHC class I-restricted fashion in tumor cells and recognized by cytotoxic T cells at tumor sites in patients with melanoma.4,5 Cayman's MART1 (N-Term) Rabbit Monoclonal Antibody (Clone RM333) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hoashi, T., Watabe, H., Muller, J., et al. MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes. The Journal of Biological Chemisty 280(14), 14006-14016 (2005).

    2. De Mazière, A.M., Muehlethaler, K., van Donselaar, E., et al. The melanocytic protein Melan-A/MART-1 has a subcellular localization distinct from typical melanosomal proteins. Traffic 3(9), 678-693 (2002).

    3. Du, J., Miller, A.J., Widlund, H.R., et al. MLANA/MART1 and SILV/PMEL17/GP100 are transcriptionally regulated by MITF in melanocytes and melanoma. Am. J. Pathol. 163(1), 333-343 (2003).

    4. Wang, F., Bade, E., Kuniyoshi, C., et al. Phase I trial of a MART-1 peptide vaccine with incomplete Freund’s adjuvant for resected high-risk melanoma. Clin. Cancer Res. 5(10), 2756-2765 (1999).

    5. Pittet, M.J., Zippelius, A., Valmori, D., et al. Melan-A/MART-1-specific CD8 T cells: From thymus to tumor. Trends Immunol. 23(7), 325-328 (2002).