For immunochemical detection of CD3ε
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CD3ε (C-Term) Rabbit Monoclonal Antibody (Clone RM344)

Item No. 32277

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CD3E
  • Cluster of Differentiation 3E
Immunogen
Peptide from the C-terminal region of human CD3ε
Clone Designation
RM344
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) CD3ε cytoplasmic domain
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

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    Product Description

    CD3 is a co-receptor for the T cell receptor (TCR), a cell surface receptor that recognizes antigens presented by MHC molecules and has a key role in the adaptive immune response.1,2 CD3 exists as a dimer composed of γ, δ, ε, or ζ subunits, which are type I transmembrane proteins encoded by distinct genes that enable TCR signal transduction.1,3 CD3ε is composed of an extracellular immunoglobulin (Ig) domain that mediates protein-protein interactions, a short membrane-proximal connecting peptide that is critical for TCR signaling, a transmembrane segment, and an intracellular immunoreceptor tyrosine-based activation motif (ITAM) that is phosphorylated by the tyrosine kinase Lck, resulting in T cell activation, proliferation, and survival.1,2,4 CD3ε forms heterodimers with CD3γ or CD3δ that interact with CD3ζζ homodimers to generate CD3 hexamers that noncovalently bind the TCR to form the CD3-TCR complex. Formulations containing murine anti-CD3ε IgG2a monoclonal antibodies have been used in the treatment of acute allograft rejection. Cayman's CD3ε (C-Term) Rabbit Monoclonal Antibody (Clone RM344) can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the cytoplasmic domain of CD3ε from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dong, D., Zheng, L., Lin, J., et al. Structural basis of assembly of the human T cell receptor–CD3 complex. Nature 573(7775), 546-552 (2019).

    2. Birnbaum, M.E., Berry, R., Hsiao, Y.-S., et al. Molecular architecture of the αβ T cell receptor–CD3 complex. Proc. Natl. Acad. Sci. USA 111(49), 17576-17581 (2014).

    3. San José, E., Sahuquillo, A.G., Bragado, R., et al. Assembly of the TCR/CD3 complex: CD3ɛ/δ and CD3ɛ/ɣ dimers associate indistinctly with both TCR ɑ and TCR β chains. Evidence for a double TCR heterodimer model. Eur. J. Immunol. 28(1), 12-21 (1998).

    4. Li, L., Guo, X., Shi, X., et al. Ionic CD3−Lck interaction regulates the initiation of T-cell receptor signaling. Proc. Natl. Acad. Sci. USA 114(29), E5891-E5899 (2017).