For immunochemical detection of spastin
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Spastin (N-Term) Rabbit Monoclonal Antibody (Clone RM346)

Item No. 32280

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Spastic Paraplegia 4
  • SPG4
Immunogen
Peptide from the N-terminal region of human spastin
Clone Designation
RM346
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC and WB
Cross Reactivity
(+) Spastin
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Product Description

    Spastin is an ATP-dependent microtubule-severing protein and a member of the ATPases associated with various cellular activities (AAA) family encoded by SPG4 in humans.1 It is composed of a hydrophobic region, microtubule-interacting and endosomal trafficking (MIT) domain, microtubule-binding domain, and an AAA ATPase domain.2,3 It is ubiquitously expressed and localized to the endoplasmic reticulum. Alternative splicing of SPG4 produces a second shorter isoform that lacks the first 87 amino acids of full-length spastin and is localized to the cytosol.4 Spastin functions as a hexamer that binds to tubulin dimers on the plus-end of microtubules and disrupts tubulin dimer interactions in an ATP-dependent manner to sever them from microtubules.5 Mutations in SPG4 are associated with autosomal dominant uncomplicated hereditary spastic paraplegia (HSP).2,4 Cayman’s Spastin (N-Term) Rabbit Monoclonal Antibody (Clone RM346) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lopes, A.T., Hausrat, T.J., Heisler, F.F., et al. Spastin depletion increases tubulin polyglutamylation and impairs kinesin-mediated neuronal transport, leading to working and associative memory deficits. PLoS Biol. 18(8), e3000820 (2020).

    2. Allison, R., Edgar, J.R., and Reid, E. Spastin MIT domain disease-associated mutations disrupt lysosomal function. Front. Neurosci. 13, 1179 (2019).

    3. Errico, A., Ballabio, A., and Rugarli, E.I. Spastin, the protein mutated in autosomal dominant hereditary spastic paraplegia, is involved in microtubule dynamics. Hum. Mol. Genet. 11(2), 153-163 (2002).

    4. Plaud, C., Joshi, V., Kajevu, N., et al. Functional differences of short and long isoforms of spastin harboring missense mutation. Dis. Model Mech. 11(9), dmm033704 (2018).

    5. Sharp, D.J., and Ross, J.L. Microtubule-severing enzymes at the cutting edge. J. Cell Sci. 125(pt 11), 2561-2569 (2012).