For immunochemical detection of TPO
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

TPO (C-Term) Rabbit Monoclonal Antibody (Clone RM368)

Item No. 32293

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Iodide Peroxidase
  • Thyroid Peroxidase
  • Thyroperoxidase
Immunogen
Peptide from the C-terminal region of human TPO
Clone Designation
RM368
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC and WB
Cross Reactivity
(+) TPO
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    Thyroid peroxidase (TPO) is a heme-containing enzyme that is essential for the biosynthesis of thyroid hormones.1,2 It exists as a homodimer where each monomer is composed of an extracellular N-terminal signal peptide and propeptide, an ectodomain containing MPO-, CCP-, and EGF-like domains, a transmembrane domain, and an intracellular C-terminal domain.2 TPO is regulated primarily at the transcriptional level by thyroid-stimulating hormone (TSH), which upregulates TPO expression. TPO is expressed by thyroid follicular cells and localizes to the apical plasma membrane in its active state.1,2 It catalyzes the oxidation of iodine, resulting in the formation of monoiodotyrosine (MIT) and diiodotyrosine (DIT) residues on thyroglobulin, a protein that functions as a scaffold for thyroid hormone biosynthesis.3 TPO subsequently catalyzes the oxidative coupling between MIT and DIT residues on thyroglobulin to produce the thyroid hormones triiodothyronine (T3) and thyroxine (T4).3 Serum TPO autoantibodies are a hallmark of autoimmune thyroid diseases, including Hashimoto's disease and Graves' disease, and have been found in patients with breast cancer.4,2 Cayman's TPO (C-Term) Rabbit Monoclonal Antibody (Clone RM368) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ruf, J., and Carayon, P. Structural and functional aspects of thyroid peroxidase. Arch. Biochem. Biophys. 445(2), 269-277 (2006).

    2. Godlewska, M., and Banga, P.J. Thyroid peroxidase as a dual active site enzyme: Focus on biosynthesis, hormonogenesis and thyroid disorders of autoimmunity and cancer. Biochimie 160, 34-45 (2019).

    3. Citterio, C.E., Targovnik, H.M., and Arvan, P. The role of thyroglobulin in thyroid hormonogenesis. Nat. Rev. Endocrinol. 15(6), 323-338 (2019).

    4. Fröhlich, E., and Wahl, R. Thyroid autoimmunity: Role of anti-thyroid antibodies in thyroid and extra-thyroidal diseases. Front. Immunol. 8, 521 (2017).