For immunochemical detection of CD163
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CD163 (N-Term) Rabbit Monoclonal Antibody (Clone RM371)

Item No. 32296

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Peptide from the N-terminal region of human CD163
Clone Designation
RM371
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) CD163
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

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    Cayman Chemical
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    Product Description

    CD163 is a member of the scavenger receptor family that has a role in inflammation.1,2 It is composed of an N-terminal ectodomain, which contains nine scavenger receptor cysteine-rich (SRCR) class B domains that mediate protein-protein interactions, a transmembrane segment, and a short cytoplasmic tail.1,3 Alternative splicing of the CD163 pre-mRNA produces several isoforms, including three variants that differ in length of the cytoplasmic tail.3 CD163 is expressed on the surface of monocytes and macrophages and used as a marker of M2 alternatively activated macrophages.1 CD163 protein levels are increased by hemoglobin, IL-6, IL-10, or glucocorticoids and decreased by LPS, IL-4, TNF-α, or IFN-γ.1,4 It binds to and internalizes haptoglobin-hemoglobin (Hp-Hb) complexes, leading to degradation of the Hp-Hb complex and production of anti-inflammatory heme metabolites.1 A soluble form of CD163 (sCD163), resulting from proteolytic cleavage of membrane-bound CD163, has been found in plasma and other tissue fluids.3 CD163+ macrophages in inflamed tissues or plasma sCD163 levels are increased in numerous acute or chronic inflammatory diseases, including sepsis, atherosclerosis, rheumatoid arthritis, and multiple sclerosis.1,2 Cayman's CD163 (N-Term) Rabbit Monoclonal Antibody (Clone RM371) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Etzerodt, A., and Moestrup, S.K. CD163 and inflammation: Biological, diagnostic, and therapeutic aspects. Antioxid. Redox Signal. 18(17), 2352-2363 (2013).

    2. Skytthe, M.K., Graversen, J.H., and Moestrup, S.K. Targeting of CD163+ macrophages in inflammatory and malignant diseases. Int. J. Mol. Sci. 21(15), 5497 (2020).

    3. Nielsen, M.J., Madsen, M., Møller, H.J., et al. The macrophage scavenger receptor CD163: Endocytic properties of cytoplasmic tail variants. J. Leukoc. Biol. 79(4), 837-845 (2006).

    4. Buechler, C., Ritter, M., Orsó, E., et al. Regulation of scavenger receptor CD163 expression in human monocytes and macrophages by pro- and antiinflammatory stimuli. J. Leukoc. Biol. 67(1), 97-103 (2000).