For immunochemical detection of MSH2
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MSH2 Rabbit Monoclonal Antibody (Clone RM375)

Item No. 32300

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • DNA Mismatch Repair Protein MSH2
  • HNPCC1
  • MutS Homolog 2
Immunogen
Recombinant peptide within 500 amino acids of the N-terminus of human MSH2
Clone Designation
RM375
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) MSH2
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    MutS homolog 2 (MSH2) is a DNA repair protein.1 It heterodimerizes with MSH6 or MSH3 to form the MutSα and MutSβ DNA mismatch recognition complexes, respectively, which are divided into five domains: a mismatch-binding domain, connector domain, α-helical lever domain, clamp domain, and an ABC-ATPase domain.1,2 It is ubiquitously expressed and localized to the nucleus.1,2 MSH2, when complexed with MSH6 or MSH3, recognizes DNA polymerase errors in replicated DNA to activate MutL and initiate DNA repair. Missense mutations in MSH2 are associated with hereditary nonpolyposis colorectal cancer (HNPCC).1,3 Polymorphisms in MSH2 are associated with an increased risk of developing prostate cancer.4 Cayman’s MSH2 Rabbit Monoclonal Antibody (Clone RM375) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kumar, D.T., Susmita, B., Judith, E., et al. Elucidating the role of interacting residues of the MSH2-MSH6 complex in DNA repair mechanism: A computational approach. Advances in Protein Chemistry and Structural Biology 325-350 (2019).

    2. Guo, J., Gu, L., Leffak, M., et al. MutSβ promotes trinucleotide repeat expansion by recruiting DNA polymerase β to nascent (CAG)n or (CTG)n hairpins for error-prone DNA synthesis. Cell Res. 26(7), 775-786 (2016).

    3. Warren, J.J., Pohlhaus, T.J., Changela, A., et al. Structure of the human MutSα DNA lesion recognition complex. Mol. Cell 26(4), 579-592 (2007).

    4. Zhen, J.T., Syed, J., Nguyen, K.A., et al. Genetic testing for hereditary prostate cancer: Current status and limitations. Cancer 124(15), 3105-3117 (2018).