For immunochemical detection of MSH6
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MSH6 Rabbit Monoclonal Antibody (Clone RM376)

Item No. 32301

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • DNA Mismatch Repair MSH6
  • MutS Homolog 6
Immunogen
Peptide corresponding to human MSH6
Clone Designation
RM376
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) MSH6
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    MutS homolog 6 (MSH6) is a DNA repair protein.1 It heterodimerizes with MSH2 to form the MutSα DNA mismatch recognition complex, which are divided into five domains: a mismatch-binding domain, connector domain, α-helical lever domain, clamp domain, and an ABC-ATPase domain.1,2 It is ubiquitously expressed and localized to the nucleus. MSH6, when complexed with MSH2, recognizes DNA polymerase errors in replicated DNA to activate MutL and initiate DNA repair. Missense mutations in MSH6 are associated with hereditary nonpolyposis colorectal cancer (HNPCC), as well as endometrial and non-hereditary colon cancers.1,3 Polymorphisms in MSH6 are associated with an increased risk of developing prostate cancer.4 Cayman’s MSH6 Rabbit Monoclonal Antibody (Clone RM376) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kumar, D.T., Susmita, B., Judith, E., et al. Elucidating the role of interacting residues of the MSH2-MSH6 complex in DNA repair mechanism: A computational approach. Advances in Protein Chemistry and Structural Biology 325-350 (2019).

    2. Guo, J., Gu, L., Leffak, M., et al. MutSβ promotes trinucleotide repeat expansion by recruiting DNA polymerase β to nascent (CAG)n or (CTG)n hairpins for error-prone DNA synthesis. Cell Res. 26(7), 775-786 (2016).

    3. Warren, J.J., Pohlhaus, T.J., Changela, A., et al. Structure of the human MutSα DNA lesion recognition complex. Mol. Cell 26(4), 579-592 (2007).

    4. Zhen, J.T., Syed, J., Nguyen, K.A., et al. Genetic testing for hereditary prostate cancer: Current status and limitations. Cancer 124(15), 3105-3117 (2018).