For immunochemical detection of BOB-1
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BOB-1/OBF-1 (C-Term) Rabbit Monoclonal Antibody (Clone RM378)

Item No. 32303

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • B cell-specific Coactivator OBF-1
  • BOB.1
  • OCA-B
  • OCT-binding Factor 1
  • POU Domain Class 2-associating Factor 1
  • POU2AF1
Immunogen
Peptide from the C-terminal region of human BOB-1
Clone Designation
RM378
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) BOB-1
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    B cell Oct Binding Factor 1 (BOB-1) is a transcription factor that has a role in B cell development and function.1 It contains a polypeptide region, a two-turn α-helix, and a disordered region.2 It is primarily expressed on B cells, with the highest expression in germinal center B cells, and transiently expressed on activated T cells.1 Its expression is induced by downstream signaling of B cell receptors, as well as IL-4, CD40 ligand (CD40L), and LPS.3 BOB-1 is a coactivator that is recruited to the POU domain of the transcription factors Oct-1 and Oct-2 and enhances their transactivation.1 Mice deficient in POU2AF1, the gene encoding BOB-1, have reduced numbers of transitional and mature B cells and lack a marginal zone B cell compartment and germinal center in the bone marrow, leading to deficits in secondary immunoglobulin isotype production.3,4 POU2AF1 is downregulated in classical, but not lymphocyte-predominant, Hodgkin lymphoma.5 Cayman’s BOB-1/OBF-1 (C-Term) Rabbit Monoclonal Antibody (Clone RM378) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sauter, P., and Matthias, P. Coactivator OBF-1 makes selective contacts with both the POU-specific domain and the POU homeodomain and acts as a molecular clamp on DNA. Mol. Cell. Biol. 18(12), 7397-7409 (1998).

    2. Chasman, D., Cepek, K., Sharp, P.A., et al. Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: Specific recognition of a protein-DNA interface. Genes Dev. 13(20), 2650-2657 (1999).

    3. Brunner, C., and Wirth, T. BOB.1/OBF.1 - A critical regulator of B cell function. Curr. Immunol. Rev. 2(1), 3-12 (2006).

    4. Kim, U., Qin, X.-F., Gong, S., et al. The B-cell-specific transcription coactivator OCA-B/OBF-1/Bob-1 is essential for normal production of immunoglobulin isotypes. Nature 383(6600), 542-547 (1996).

    5. Stein, H., Marafioti, T., Foss, H.-D., et al. Down-regulation of BOB.1/OBF.1 and Oct2 in classical Hodgkin disease but not in lymphocyte predominant Hodgkin disease correlates with immunoglobulin transcription. Blood 97(2), 496-501 (2001).