Terminal Deoxynucleotidyl Transferase Rabbit Monoclonal Antibody (Clone RM379)

Terminal deoxynucleotidyl transferase (TdT) is a template-independent member of the X family of DNA polymerases and encoded by DNTT in humans.^1,2 It is composed of finger, thumb, palm, and index finger subdomains, as well as a lariat-like loop that prevents interaction with the DNA template strand.² TdT is expressed in lymphoid tissues, such as the thymus and bone marrow, as well as lymphocyte progenitor cells, and localized to the nucleus.^2,3 During V(J)D recombination, TdT randomly adds nucleotides to sites of double-strand DNA breaks, prior to non-homologous end-joining, increasing the variability of the non-templated nucleotide region of the recombined gene segments.^1,2 DNTT expression is associated with remission and increased median survival in patients with acute lymphoblastic leukemia.³ Cayman’s Terminal Deoxynucleotidyl Transferase Rabbit Monoclonal Antibody (Clone RM379) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

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1. Sarac, I., and Hollenstein, M. Terminal deoxynucleotidyl transferase in the synthesis and modification of nucleic acids. Chembiochem 20(7), 860-871 (2018).

2. Motea, E.A., and Berdis, A.J. Terminal deoxynucleotidyl transferase: The story of a misguided DNA polymerase. Biochim. Biophys. Acta 1804(5), 1151-1166 (2010).

3. Rambotti, P., and Davis, S. Biochemical markers in lymphoproliferative diseases. Crit. Rev. Oncol. Hematol. 2(4), 297-321 (1985).