For immunochemical detection of Ki-67
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Ki-67 Rabbit Monoclonal Antibody (Clone RM360)

Item No. 32309

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Antigen Ki-67
  • Marker of Proliferation Ki-67
Immunogen
Peptide corresponding to the internal region of human Ki-67
Clone Designation
RM360
Formulation (Request formulation change)
100 µl of Protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) Ki-67
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    100 µl

    $411

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    Cayman Chemical
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    Product Description

    Ki-67 is a non-histone nuclear and nucleolar protein and marker of cell proliferation encoded by MKI67 in humans.1,2 It is composed of an N-terminal forkhead-associated domain, a protein phosphatase 1-binding domain, a central conserved domain, 16 concatenated direct repeats, and six C-terminal leucine-arginine rich motif repeats.2 Ki-67 expression and localization is cell cycle-specific. It is localized to the perinuclear region during G1, the fibrillary component of the nucleolus during interphase, and the perichromosomal layer during chromatin condensation, with Ki-67 levels increasing from late G1 phase to S phase and peaking at mitosis.1 Ki-67 is associated with proliferative activity of intrinsic cell populations in tumors and is commonly used as a marker of tumor aggressiveness in patients with breast, lung, prostate, cervical, or CNS cancers.3 Ki-67 is also commonly used as a marker of adult neurogenesis.4 Cayman’s Ki-67 Rabbit Monoclonal Antibody (Clone RM360) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Menon, S.S., Guruvayoorappan, C., Sakthivel, K.M., et al. Ki-67 protein as a tumour proliferation marker. Clin. Chim. Acta 491, 39-45 (2019).

    2. Sun, X., and Kaufman, P.D. Ki-67: More than a proliferation marker. Chromosoma 127(2), 175-186 (2018).

    3. Li, L.T., Jiang, G., Chen, Q., et al. Ki67 is a promising molecular target in the diagnosis of cancer (review). Mol. Med. Rep. 11(3), 1566-1572 (2015).

    4. Kee, N., Sivalingam, S., and Wojtowicz, J.M. The utility of Ki-67 and BrdU as proliferative markers of adult neurogenesis. J. Neurosci. Methods 115(1), 97-105 (2002).