For immunochemical detection of CD33
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

CD33 (C-Term) Rabbit Monoclonal Antibody (RM398)

Item No. 32317

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cluster of Differentiation 33
  • Myeloid Cell Surface Antigen CD33
  • Sialic Acid-binding Ig-like Lectin 3
  • Siglec-3
Immunogen
Peptide from the C-terminal region of CD33
Clone Designation
RM398
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) CD33
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    CD33 is a transmembrane receptor and member of the sialic acid-binding immunoglobulin-type (Ig-type) lectin (SIGLEC) family.1 It is composed of N-terminal extracellular Ig-like variable (IgV) and Ig-like constant 2 (IgC2) domains, as well as intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM) and ITIM-like (ITL) domains. CD33 has two isoforms formed via alternative splicing, a full-length form and CD33m, which lacks the IgV-like domain.2 The full-length form is expressed on myeloid progenitor cells and in microglia while CD33m is found in myeloid cells and lymphocytes.1,2 CD33 is an inhibitory receptor activated by binding of sialic acid-containing molecules, such as glycolipids and glycoproteins, to the IgV domain, which stimulates phosphorylation of tyrosine in the ITIM domain, leading to recruitment of Src homology 2 domain-containing phosphatases (SHPs) and internalization of the CD33-ligand complex.3 Signaling downstream of CD33 inhibits phagocytosis and cytokine release from immune cells, as well as regulates immune cell growth and survival. CD33 protein is found on patient-derived acute myeloid leukemia (AML) progenitor cells but not non-cancerous stem cells.4 A SNP in CD33 is associated with, and considered a risk factor for, Alzheimer’s disease.1 Cayman’s CD33 (C-Term) Rabbit Monoclonal Antibody (RM398) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Estus, S., Shaw, B.C., Devanney, N., et al. Evaluation of CD33 as a genetic risk factor for Alzheimer’s disease. Acta Neuropathol. 138(2), 187-199 (2019).

    2. Pérez-Oliva, A.B., Martínez-Esparza, M., Vicente-Fernández, J.J., et al. Epitope mapping, expression and post-translational modifications of two isoforms of CD33 (CD33M and CD33m) on lymphoid and myeloid human cells. Glycobiology 21(6), 757-770 (2011).

    3. Zhao, L. CD33 in Alzheimer’s disease - biology, pathogenesis, and therapeutics: A mini-review. Gerontology 65(4), 323-331 (2109).

    4. Laszlo, G.S., Estey, E.H., and Walter, R.B. The past and future of CD33 as therapeutic target in acute myeloid leukemia. Blood Rev. 28(4), 143-153 (2014).