For immunochemical detection of ZAP-70
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ZAP-70 (N-Term) Rabbit Monoclonal Antibody (RM408)

Item No. 32334

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Peptide from the N-terminal region of human ZAP-70
Clone Designation
RM408
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) ZAP-70
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    ZAP-70 is a tyrosine kinase that mediates T cell receptor (TCR) signaling.1 It is composed of two tandem Src homology 2 (SH2) domains, which are critical for TCR-mediated intracellular signal transduction, a linker region containing numerous tyrosine residues subject to phosphorylation, and a kinase domain. ZAP-70 is expressed mainly by T cells and natural killer (NK) cells but is also expressed by certain subsets of malignant B cells.2 Following TCR stimulation, ZAP-70 is recruited from the cytoplasm to phosphorylated TCR ζ chain immunoreceptor tyrosine-based activation motifs (ITAMs), where it is phosphorylated and activated by the tyrosine kinase LCK.1,3 ZAP-70 phosphorylates the adaptor proteins LAT and SLP-76, which activate a variety of intracellular signaling pathways that lead to T cell activation, proliferation, and differentiation.1,3 ZAP70 loss-of-function mutations are associated with ZAP-70 deficiency, a SCID characterized by the absence of functional T cells and recurrent infections.1,4 Cayman's ZAP-70 (N-Term) Rabbit Monoclonal Antibody (RM408) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wang, H., Kadlecek, T.A., Au-Yeung, B.B., et al. ZAP-70: An essential kinase in T-cell signaling. Cold Spring Harb. Perspect. Biol. 2(5), a002279 (2010).

    2. Chen, J., Moore, A., and Ringshausen, I. ZAP-70 shapes the immune microenvironment in B cell malignancies. Front Oncol. 10, 595832 (2020).

    3. Au-Yeung, B.B., Shah, N.H., Shen, L., et al. ZAP-70 in signaling, biology, and disease. Annu. Rev. Immunol. 26, 127-156 (2018).

    4. Sharifinejad, N., Jamee, M., Zaki-Dizaji, M., et al. Clinical, immunological, and genetic features in 49 patients with ZAP-70 deficiency: A systematic review. Front Immunol. 11, 831 (2020).