For immunochemical detection of HP-NAP
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Helicobacter pylori NAP Rabbit Monoclonal Antibody (Clone RM414)

Item No. 32345

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H. pylori Neutrophil-activating Protein
Immunogen
Proteins purified from H. pylori
Clone Designation
RM414
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA
Cross Reactivity
(+) HP-NAP
Species Reactivity
(+) H. pylori
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $411

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    Cayman Chemical
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    Product Description

    Helicobacter pylori (H. pylori) is a Gram-negative bacterium that can infect and colonize the stomach, leading to chronic gastritis, stomach inflammation and oxidative stress, peptic ulcer disease, and gastric cancer.1 H. pylori neutrophil-activating protein (HP-NAP) is a virulence factor and member of the DNA-protecting proteins from starved cells (Dps) family.2 It is composed of 12 identical monomers each containing four α-helices.3 It activates neutrophils and increases their infiltration into the gastric mucosa by increasing the production of reactive oxygen species (ROS), secretion of myeloperoxidase (MPO), and release of cytokines from a variety of host immune cells.2 HP-NAP protects H. pylori from bacterial DNA damage induced by this oxidative stress. It also induces a cytotoxic Th1 response in T cells in vitro and is a toll-like receptor 2 (TLR2) agonist that activates NF-κB in HEK293 cells.4 HP-NAP is an iron-binding protein, and H. pylori strains isolated from patients with iron-deficiency anemia have a SNP in napA, the gene encoding HP-NAP, and an increase in iron uptake compared with strains not containing the SNP.3,5 HP-NAP is immunogenic in humans, and oral recombinant HP-NAP administration is protective against H. pylori infection in mice.6 Cayman’s Helicobacter pylori NAP Rabbit Monoclonal Antibody (Clone RM414) can be used for ELISA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Chang, A.H., and Parsonnet, J. Role of bacteria in oncogenesis. Clin. Microbiol. Rev. 23(4), 837-857 (2010).

    2. Fu, H.-W. Helicobacter pylori neutrophil-activating protein: From molecular pathogenesis to clinical applications. World J. Gastroenterol. 20(18), 5294-5301 (2014).

    3. Tonello, F., Dundon, W.G., Satin, B., et al. The Helicobacter pylori neutrophil-activating protein is an iron-binding protein with dodecameric structure. Mol. Microbiol. 34(2), 238-246 (1999).

    4. Amedei, A., Cappon, A., Codolo, G., et al. The neutrophil-activating protein of Helicobacter pylori promotes Th1 immune responses. J. Clin. Invest. 116(4), 1092-1101 (2006).

    5. Yokota, S.-I., Toita, N., Yamamoto, S., et al. Positive relationship between a polymorphism in Helicobacter pylori neutrophil-activating protein a gene and iron-deficiency anemia. Helicobacter 18(2), 112-116 (2013).

    6. Satin, B., Del Giudice, G., Della Bianca, V., et al. The neutrophil-activating protein (HP-NAP) of Helicobacter pylori is a protective antigen and a major virulence factor. J. Exp. Med. 191(9), 1467-1476 (2000).