For immunochemical detection of Igλ light chain
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Igλ Light Chain (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM110)

Item No. 32346

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin λ Light Chain
Immunogen
Mouse IgMλ
Clone Designation
RM110
Formulation (Request formulation change)
50 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, WB (non-reducing conditions)
Cross Reactivity
(+) Igλ light chain(-) Igκ light chain(-) Human IgG(-) Goat IgG(-) Rat IgG
Species Reactivity
(+) Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $411

    Add
    Product Description

    Igλ light chain is one type of light chain found in immunoglobulins, which are part of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1 Immunoglobulins are produced by B cells and later secreted by plasma cells as antibodies.2 They are composed of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.3 Mammalian immunoglobulins contain either Igκ or Igλ light chains each of which are composed of a constant and variable domain.2,4 The ratio of Igκ to Igλ light chain containing antibodies varies between species, with ratios of 20:1, 2:1, and 1:20 in mice, humans, and cattle, respectively. In systemic amyloidosis, a clonal population of plasma cells produces light chains that form amyloid fibrils, and the type of free light chains (FLCs) produced is predominantly Igλ with an Igκ to Igλ ratio of 1:3 or, in amyloidosis patients with nephrotic-range proteinuria, 1:5.5 Cayman’s Igλ Light Chain (mouse) Monoclonal Antibody - Biotinylated (RM110) can be used for ELISA and Western blot (WB; non-reducing conditions) applications. The antibody recognizes the Igλ light chain from mouse samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.W., Jr., and Cavicini, L. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125(2 Suppl. 2), S41-S52 (2010).

    2. Esparvarinha, M., Nickho, H., Mohammadi, H., et al. The role of free kappa and lambda light chains in the pathogenesis and treatment of inflammatory diseases. Biomed. Pharmacother. 91, 632-644 (2017).

    3. Vaillant, A.A.J., and Ramphul, K. Immunoglobulin. 2020, (2020).

    4. Janeway, C.A., Jr., Travers, P., Walport, M., et al. Antigen recognition by B-cell and T-cell receptors. Immunobiology: The immune system in health and disease 103-107 (2005).

    5. Gertz, M.A., Lacy, M.Q., and Dispenzieri, A. Immunoglobulin light chain amyloidosis and the kidney. Kidney Int. 61(1), 1-9 (2002).