For immunochemical detection of Igκ light chain
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Igκ Light Chain (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM103)

Item No. 32347

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin κ Light Chain
Immunogen
Mouse IgG
Clone Designation
RM103
MW
~150 and ~25 kDa for non-reduced and reduced Igκ, respectively
Formulation (Request formulation change)
50 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, WB
Cross Reactivity
(+) Igκ light chain(-) Mouse Igλ light chain(-) Human IgG(-) Goat IgG(-) Rat IgG
Species Reactivity
(+) Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $411

    Add
    Cayman Chemical
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    Product Description

    Igκ light chain is one type of light chain found in immunoglobulins, which are part of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1 Immunoglobulins are produced by B cells and later secreted by plasma cells as antibodies.2 They are composed of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.3 Mammalian immunoglobulins contain either Igκ or Igλ light chains, each of which are composed of a constant and variable domain.4 The ratio of Igκ to Igλ light chain-containing antibodies varies between species, with ratios of 20:1, 2:1, and 1:20 in mice, humans, and cattle, respectively. Igκ and Igλ free light chains (FLCs) are produced during immunoglobulin synthesis, and accumulation of these FLCs, primarily Igκ, is associated with various disorders, including light-chain deposition disease, multiple myeloma, rheumatoid arthritis, diabetic nephropathy, and systemic lupus erythematosus (SLE).2,5,6 Cayman's Igκ Light Chain (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM103) can be used for ELISA and Western blot (WB) applications. The antibody recognizes the non-reduced and reduced Igκ light chain from mouse samples at approximately 150 and 25 kDa, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.W., Jr., and Cavicini, L. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125(2 Suppl. 2), S41-S52 (2010).

    2. Esparvarinha, M., Nickho, H., Mohammadi, H., et al. The role of free kappa and lambda light chains in the pathogenesis and treatment of inflammatory diseases. Biomed. Pharmacother. 91, 632-644 (2017).

    3. Vaillant, A.A.J., and Ramphul, K. Immunoglobulin. 2020, (2020).

    4. Janeway, C.A., Jr., Travers, P., Walport, M., et al. Antigen recognition by B-cell and T-cell receptors. Immunobiology: The immune system in health and disease 103-107 (2005).

    5. Jimenez-Zepeda, V.H. Light chain deposition disease: Novel biological insights and treatment advances. Int. J. Lab. Hematol. 34(4), 347-355 (2012).

    6. Sannier, A., Hanouna, G., Daugas, E., et al. IgA kappa light and heavy chain deposition disease in multiple myeloma. Br. J. Haematol. 183(1), 13 (2018).