For immunochemical detection of IgG2aκ
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IgG2aκ (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM107)

Item No. 32351

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin G2aκ
Immunogen
Mouse IgG
Clone Designation
RM107
Formulation (Request formulation change)
50 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA and WB (non-reducing conditions)
Cross Reactivity
(+) IgG2aκ(-) Mouse IgG2aλ(-) Mouse IgG1(-) Mouse IgG3(-) Mouse IgM(-) Mouse IgA(-) Mouse IgE(-) Human IgG(-) Goat IgG(-) Rat IgG
Species Reactivity
(+) Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $411

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    Cayman Chemical
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    Product Description

    Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1 It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.1,2,3 IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.4 IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.2 IgG exists as four isotypes in mice: IgG1, IgG2b, IgG3, and, in a strain-specific manner, IgG2a or IgG2c.5,6 In vivo, class switching to the IgG2a isotype can happen via IFN-γ-dependent and -independent mechanisms, with the former resulting from the cognate interaction of B cells with T helper 1 (Th1) cells.7 IgG2a is the predominant isotype produced in response to infection with DNA or RNA viruses in mice.8 Mammalian immunoglobulins contain either Igκ or Igλ light chains, each of which are composed of a constant and variable domain.9 Cayman's IgG2aκ (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM107) can be used for ELISA and Western blot (WB; non-reducing conditions). The antibody recognizes the Fab region of IgG2aκ from mouse samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.W., Jr., and Cavicini, L. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125(2 Suppl. 2), S41-S52 (2010).

    2. Vidarsson, G., Dekkers, G., and Rispens, T. IgG subclasses and allotypes: From structure to effector functions. Front. Immunol. 5, 520 (2014).

    3. Mayumi, M., Kuritani, T., Kubagawa, H.M., et al. IgG subclass expression by human B lymphocytes and plasma cells: B lymphocytes precommitted to IgG subclass can be preferentially induced by polyclonal mitogens with T cell help. J. Immunol. 130(2), 671-677 (1983).

    4. Vaillant, A.A.J., and Ramphul, K. Immunoglobulin. 2020, (2020).

    5. Collins, A.M. IgG subclass co-expression brings harmony to the quartet model of murine IgG function. Immunol. Cell Biol. 94(10), 949-954 (2016).

    6. Martin, R.M., Brady, J.L., and Lew, A.M. The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice. J. Immunol. Methods 212(2), 187-192 (1998).

    7. Jegerlehner, A., Maurer, P., Bessa, J.M., et al. TLR9 signaling in B cells determines class switch recombination to IgG2a. J. Immunol. 178(4), 2415-2420 (2007).

    8. Coutlelier, J.P., Van Der Logt, J.T.M., Heessen, F.W.A., et al. IgG2a restriction of murine antibodies elicited by viral infections. J. Exp. Med. 165(1), 64-69 (1987).

    9. Janeway, C.A., Jr., Travers, P., Walport, M., et al. Antigen recognition by B-cell and T-cell receptors. Immunobiology: The immune system in health and disease 103-107 (2005).