For immunochemical detection of IgA
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IgA (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM220)

Item No. 32355

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin A
Immunogen
Mouse IgA
Clone Designation
RM220
Formulation (Request formulation change)
50 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, WB (non-reducing conditions)
Cross Reactivity
(+) IgA(-) Mouse IgG1(-) IgG2a(-) IgG2b(-) IgG2c(-) IgG3(-) IgM(-) IgE(-) Human IgA
Species Reactivity
(+) Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $411

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    Cayman Chemical
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    Product Description

    Immunoglobulin A (IgA) is a member of the immunoglobulin superfamily of glycoproteins with roles in host defense against intestinal pathogens and both quantitative and qualitative control of host commensal microbiota composition.1,2 It is produced by B cells and later secreted by plasma cells and is the most abundant antibody on mucosal surfaces that comprises at least 70% of all Ig produced in mice. Mouse IgA consists of two heavy chains of approximately 53.5 kDa each and two light chains of approximately 25 kDa each.3 Unlike human IgA, mouse IgA exists as a single isotype and is primarily found as a dimer that lacks the disulfide bonds between the light and heavy chains present in other Ig classes.3,4 Production of IgA is induced in the gut only in animals containing intestinal microbes, and the number of IgA-producing plasma cells is reduced in germ-free mice.1 IgA-deficient mice exhibit increased lethality compared with wild-type mice in a model of influenza infection, as well as reduced parasite clearance in a model of G. muris infection. However, IgA deficiency does not affect clearance of vaginal infection with herpes simplex virus 2 (HSV-2), indicating redundancy in pathogen protection with compensation by antibodies of other isotypes or innate immune mechanisms at mucosal surfaces. Cayman's IgA (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM220) can be used for ELISA and Western blot (WB; non-reducing conditions) applications. The antibody recognizes IgA from mouse samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Macpherson, A.J., McCoy, K.D., Johansen, F.-E., et al. The immune geography of IgA induction and function. Mucosal Immunol. 1(1), 11-22 (2008).

    2. Mathias, A., Pais, B., Favre, L., et al. Role of secretory IgA in the mucosal sensing of commensal bacteria. Gut Microbes 5(6), 688-695 (2014).

    3. Grey, H.M., Sher, A., and Shalitin, N. The subunit structure of mouse IgA. J. Immunol. 105(1), 75-84 (1970).

    4. de Sousa-Pereira, P., and Woof, J.M. IgA: Structure, function, and developability. Antibodies (Basel) 8(4), 57 (2019).