For immunochemical detection of IgG2c
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IgG2c (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM223)

Item No. 32357

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin G2c
Immunogen
Mouse IgG2c
Clone Designation
RM223
Formulation (Request formulation change)
50 µg protein A-purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, WB (non-reducing conditions)
Cross Reactivity
(+) IgG2c(-) Mouse IgG1(-) Mouse IgG2a(-) Mouse IgG2b(-) Mouse IgG3(-) Mouse IgM(-) Mouse IgA(-) Mouse IgE(-) Human IgG(-) Rat IgG
Species Reactivity
(+) Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $411

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    Cayman Chemical
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    Product Description

    Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1 It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.1,2,3 IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.4 IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.2 IgG exists as four isotypes in mice: IgG1, IgG2b, IgG3, and, in a strain-specific manner, IgG2a or IgG2c.5,6 IgG2c is encoded by Ighg2c and is expressed in certain inbred mouse strains, such as C57BL/6, C57BL/10, SJL, and NOD mice.6 Class switching to the IgG2c isotype occurs via IFN-γ stimulation during the early immune response.7 IgG2c binds to the high-affinity Igγ Fc receptor I (FcγRI) on dendritic cells and the low-affinity FcγRIII and FcγRIV, which are expressed on a variety of immune cells, and is involved in complement activation.8,9 Cayman's IgG2c (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM223) can be used for ELISA and Western blot (WB; non-reducing conditions) applications. The antibody recognizes the Fc region of IgG2c from mouse samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.W., Jr., and Cavicini, L. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125(2 Suppl. 2), S41-S52 (2010).

    2. Vidarsson, G., Dekkers, G., and Rispens, T. IgG subclasses and allotypes: From structure to effector functions. Front. Immunol. 5, 520 (2014).

    3. Mayumi, M., Kuritani, T., Kubagawa, H.M., et al. IgG subclass expression by human B lymphocytes and plasma cells: B lymphocytes precommitted to IgG subclass can be preferentially induced by polyclonal mitogens with T cell help. J. Immunol. 130(2), 671-677 (1983).

    4. Vaillant, A.A.J., and Ramphul, K. Immunoglobulin. 2020, (2020).

    5. Collins, A.M. IgG subclass co-expression brings harmony to the quartet model of murine IgG function. Immunol. Cell Biol. 94(10), 949-954 (2016).

    6. Martin, R.M., Brady, J.L., and Lew, A.M. The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice. J. Immunol. Methods 212(2), 187-192 (1998).

    7. Barr, T.A., Brown, S., Mastroeni, P., et al. B cell intrinsic MyD88 signals drive IFN-γ production from T cells and control switching to IgG2c. J. Immunol. 183(2), 1005-1012 (2009).

    8. Strait, R.T., Thornton, S., and Finkelman, F.D. Cγ1 deficiency exacerbates collagen-induced arthritis. Arthritis Rheumatol. 68(7), 1780-1787 (2016).

    9. Bruhns, P. Properties of mouse and human IgG receptors and their contribution to disease models. Blood 119(24), 5640-5649 (2012).