A fluorescent probe for amyloid fibrils
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Thioflavin T

Item No. 32553

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-[4-(dimethylamino)phenyl]-3,6-dimethyl-benzothiazolium, monochloride
CAS Number
2390-54-7
Synonyms
  • ThT
Molecular Formula
C17H19N2S • Cl
Formula Weight
318.9
Purity
≥90%
Emission
445, 482 nm
Excitation
385, 450 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 1 mg/mlEthanol: 1 mg/mlEthanol:PBS (pH 7.2) (1:3): 0.3 mg/mlPBS (pH 7.2): Slightly soluble
λmax
417 nm
SMILES
CN(C)C(C=C1)=CC=C1C2=[N+](C)C3=C(C=C(C)C=C3)S2.[Cl-]
InChi Code
InChI=1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1
InChi Key
JADVWWSKYZXRGX-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    10 g

    $44

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    25 g

    $72

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    50 g

    $130

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    100 g

    $238

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    Product Description

    Thioflavin T is a fluorescent probe for amyloid fibrils.1 It displays excitation/emission maxima of 385/445 nm, respectively, and exhibits an increase in fluorescence intensity and a shift in excitation/emission maxima to 450/482 nm upon binding to amyloid fibrils.1,2 The fluorescence intensity is also pH dependent, increasing in intensity as the pH increases.2 Thioflavin T binds to different types of amyloid fibrils with varying affinity, over two orders of magnitude, depending on type and binding mode.3 It has been used with fluorescence microscopy to visualize brain amyloid fibril plaques in a rat model of Alzheimer's disease.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Biancalana, M., and Koide, S. Molecular mechanism of Thioflavin-T binding to amyloid fibrils. Biochim. Biophys. Acta 1804(7), 1405-1412 (2010).

    2. Naiki, H., Higuchi, K., Hosokawa, M., et al. Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye, thioflavin T. Anal. Biochem. 177(2), 244-249 (1989).

    3. Sulatskaya, A.I., Kuznetsova, I.M., Belousov, M.V., et al. Stoichiometry and affinity of thioflavin T binding to Sup35p amyloid fibrils. PLoS One 11(5), e0156314 (2016).

    4. Alimohammadi-Kamalabadi, M., Eshraghian, M., Zarindast, M.-R., et al. Effect of creatine supplementation on cognitive performance and apoptosis in a rat model of amyloid-beta-induced Alzheimer’s disease. Iran J. Basic Med. Sci. 19(11), 1159-1165 (2016).