For immunochemical detection of 12-LO (leukocyte-type)
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12-Lipoxygenase (mouse) Polyclonal Antibody

Item No. 32822

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Alox12
  • Arachidonate 12-lipoxygenase
  • 12-LO
  • 12-LOX
Immunogen
Full-length recombinant mouse 12-lipoxygenase (leukocyte-type)
Formulation (Request formulation change)
500 µg of protein A-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
ELISA and WB
Cross Reactivity
(+) Human 15-Lipoxygenase-2
Species Reactivity
(+) Mouse(+) Porcine
UniProt Accession №
P39654
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    500 µg

    $347

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    Product Description

    12-Lipoxygenase (12-LO) is one of several LOs that catalyzes the peroxidation of polyunsaturated fatty acids in a position-specific manner.1 There are four 12-LO subtypes, encoded by distinct genes, that can be categorized according to their tissue expression in mice.2 Leukocyte-type 12-LO is encoded by Alox15 in mice and shares 74% sequence identity with human ALOX15 but, in contrast to its human ortholog, encodes a 12-lipoxygenating ALOX15 isoform.3,4,5 Leukocyte-type 12-LO exists as a single polypeptide chain composed of an N-terminal regulatory domain and a C-terminal catalytic domain.1 It catalyzes the oxidation of arachidonic acid (Item Nos. 90010 | 90010.1 | 10006607) to 12(S)-HpETE (Item No. 44570) and, to a lesser extent, 15(S)-HpETE (Item No. 44720), which are further reduced to 12(S)-HETE (Item No. 34570) and 15(S)-HETE (Item No. 34720), respectively.1,6,7 Other substrates of leukocyte-type 12-LO include eicosapentaenoic acid, docosahexaenoic acid, linoleic acid, and γ-linolenic acid, which are oxygenated at various positions in a substrate-dependent manner.8 Leukocyte-type 12-LO is constitutively expressed at high levels in resident peritoneal macrophages, and its activity is increased by the Th2 cytokines IL-4 and IL-13 in vitro.5 Alox15-/- mice are protected from increases in systolic blood pressure in a model of hypertension induced by L-NAME (Item No. 80210).9 Cayman's 12-Lipoxygenase (mouse) Polyclonal Antibody can be used for ELISA and Western blot (WB) applications. The antibody recognizes 12-LO (leukocyte-type) at ~73 kDa from porcine and mouse samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kühn, H., Banthiya, S., and van Leyen, K. Mammalian lipoxygenases and their biological relevance. Biochim. Biophys. Acta 1851(4), 308-330 (2015).

    2. Kühn, H., and O'Donnell, V.B. Inflammation and immune regulation by 12/15-lipoxygenases. Prog. Lipid Res. 45(4), 334-356 (2006).

    3. Ivanov, I., Kühn, H., and Heydeck, D. Structural and functional biology of arachidonic acid 15-lipoxygenase-1 (ALOX15). Gene 573(1), 1-32 (2015).

    4. Adel, S., Karst, F., González-Lafont, À., et al. Evolutionary alteration of ALOX15 specificity optimizes the biosynthesis of antiinflammatory and proresolving lipoxins. Proc. Natl. Acad. Sci. USA 113(30), E4266–E4275 (2016).

    5. Kühn, H., Gehring, T., Schröter, A., et al. Cytokine-dependent expression regulation of ALOX15. J. Cytokine Biol. 1(2), 1000106 (2016).

    6. Mashima, R., and Okuyama, T. The role of lipoxygenases in pathophysiology; new insights and future perspectives. Redox Biol. 6, 297-310 (2015).

    7. Chen, X.-S., Kurre, U., Jenkins, N.A., et al. cDNA cloning, expression, mutagenesis of C-terminal isoleucine, genomic structure, and chromosomal localizations of murine 12-lipoxygenases. The Journal of Biological Chemisty 269(19), 13979-13987 (1994).

    8. Kutzner, L., Goloshchapova, K., Heydeck, D., et al. Mammalian ALOX15 orthologs exhibit pronounced dual positional specificity with docosahexaenoic acid. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1862(7), 666-675 (2017).

    9. Kriska, T., Cepura, C., Magier, D., et al. Mice lacking macrophage 12/15-lipoxygenase are resistant to experimental hypertension. Am. J. Physiol. Heart Circ. Physiol. 302(11), H2428-H2438 (2012).