A peptide fusion tag
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Product Type

Biochemicals
Peptides

DYKDDDDK Peptide (trifluoroacetate salt)

Item No. 33198

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
L-aspartyl-L-tyrosyl-L-lysyl-L-aspartyl-L-aspartyl-L-aspartyl-L-aspartyl-L-lysine, trifluoroacetate salt
Synonyms
  • DYKDDDDK Epitope
  • DYKDDDDK Octapeptide
  • DYKDDDDK Tag
Molecular Formula
C41H60N10O20 • XCF3COOH
Formula Weight
1013.0
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 10 mg/mlPBS (pH 7.2): 10 mg/ml
SMILES
OC(C=C1)=CC=C1C[C@H](NC([C@@H](N)CC(O)=O)=O)C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@H](C(O)=O)CCCCN)=O)=O)=O)=O)=O)=O.OC(C(F)(F)F)=OOC(C=C1)=CC=C1C[C@H](NC([C@@H](N)CC(O)=O)=O)C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@H](C(O)=O)CCCCN)=O)=O)=O)=O)=O)=O.OC(C(F)(F)F)=O
InChi Code
InChI=1S/C41H60N10O20.C2HF3O2/c42-11-3-1-5-22(45-36(65)24(13-19-7-9-20(52)10-8-19)47-34(63)21(44)14-29(53)54)35(64)48-26(16-31(57)58)38(67)50-28(18-33(61)62)40(69)51-27(17-32(59)60)39(68)49-25(15-30(55)56)37(66)46-23(41(70)71)6-2-4-12-43;3-2(4,5)1(6)7/h7-10,21-28,52H,1-6,11-18,42-44H2,(H,45,65)(H,46,66)(H,47,63)(H,48,64)(H,49,68)(H,50,67)(H,51,69)(H,53,54)(H,55,56)(H,57,58)(H,59,60)(H,61,62)(H,70,71);(H,6,7)/t21-,22-,23-,24-,25-,26-,27-,28-;/m0./s1
InChi Key
DODSASRCFZBKPJ-ZKDQGCNTSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $72

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    10 mg

    $129

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    25 mg

    $304

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    Product Description

    DYKDDDDK peptide is a synthetic octapeptide and fusion tag.1,2 It can be added to the N- or C-terminus of proteins or inserted into the protein sequence and later cleaved using enteropeptidase. Due to its hydrophilicity, it has a low potential to interfere with protein folding or activity. DYKDDDDK peptide has commonly been used as an epitope tag on recombinant proteins to facilitate their detection and purification by DYKDDDDK-specific antibodies in vitro and in vivo.1,2,3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hopp, T.P., Prickett, K.S., Price, V.L., et al. A short polypeptide marker sequence useful for recombinant protein identification and purification. Nat. Biotechnol. 6, 1204-1210 (1988).

    2. Einhauer, A., and Jungbauer, A. The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteins. J. Biochem. Biophys. Methods 49(1-3), 455-465 (2001).

    3. Ichiki, T., Koga, T., Okuno, T., et al. Modulation of leukotriene B4 receptor 1 signaling by receptor for advanced glycation end products (RAGE). The FASEB Journal 30(5), 1811-1822 (2016).

    4. Ikeda, K., Koga, T., Sasaki, F., et al. Generation and characterization of a human-mouse chimeric high-affinity antibody that detects the DYKDDDDK FLAG peptide. Biochem. Biophys. Res. Commun. 486(4), 1077-1082 (2017).