An amine-reactive biotinylation reagent
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Sulfo-NHS-Biotin

Item No. 33362

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanoic acid, 2,5-dioxo-3-sulfo-1-pyrrolidinyl ester, monosodium salt
CAS Number
194041-65-1
Synonyms
  • N-Hydroxysulfosuccinimide-Biotin
Molecular Formula
C14H18N3O8S2 • Na
Formula Weight
443.4
Purity
≥90%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 20 mg/mlPBS (pH 7.2): 10 mg/ml
SMILES
O=C(ON1C(CC(S(=O)([O-])=O)C1=O)=O)CCCC[C@@H]2SC[C@@](N3)([H])[C@]2([H])NC3=O.[Na+]
InChi Code
InChI=1S/C14H19N3O8S2.Na/c18-10-5-9(27(22,23)24)13(20)17(10)25-11(19)4-2-1-3-8-12-7(6-26-8)15-14(21)16-12;/h7-9,12H,1-6H2,(H2,15,16,21)(H,22,23,24);/q;+1/p-1/t7-,8-,9?,12-;/m0./s1
InChi Key
DREOJRVDBCALEG-WCYHLEDJSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    10 mg

    $40

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    25 mg

    $93

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    50 mg

    $132

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    100 mg

    $228

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    Product Description

    Sulfo-NHS-biotin is a water-soluble and amine-reactive biotinylation reagent.1,2 It is commonly used in the biotin labeling of amine-containing proteins, antibodies, and other molecules.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Polli, J.W., and Billingsley, M.L. Preparation, characterization and biological properties of biotinylated derivatives of calmodulin. Biochem J. 275 (Pt 3), 733-743 (1991).

    2. Cohen, L., and Walt, D.R. Evaluation of antibody biotinylation approaches for enhanced sensitivity of Single Molecule Array (Simoa) immunoassays. Bioconjug. Chem. 29(10), 3452-3458 (2018).