A fluorescent probe for RNA
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DFHBI 1T

Item No. 33517

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(5Z)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
CAS Number
1539318-36-9
Molecular Formula
C13H9F5N2O2
Formula Weight
320.2
Purity
≥98%
Emission
501 nm
Excitation
447 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlDMSO:PBS (pH 7.2) (1:4): 0.20 mg/mlEthanol: 1 mg/ml
λmax
363 nm
SMILES
FC(C=C1/C=C2N=C(C)N(CC(F)(F)F)C\2=O)=C(C(F)=C1)O
InChi Code
InChI=1S/C13H9F5N2O2/c1-6-19-10(12(22)20(6)5-13(16,17)18)4-7-2-8(14)11(21)9(15)3-7/h2-4,21H,5H2,1H3/b10-4-
InChi Key
AWYCLBWNRONMQC-WMZJFQQLSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

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    5 mg

    $134

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    10 mg

    $242

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    25 mg

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    Product Description

    DFHBI 1T is a fluorescent probe for RNA.1,2 It displays excitation/emission maxima of 447/501 nm, respectively, and fluoresces upon binding to RNA aptamers, such as Broccoli, Spinach, or Spinach2. DFHBI 1T has been used to image RNA in live cells.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Song, W., Strack, R.L., Svensen, N., et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J. Am. Chem. Soc. 136(4), 1198-1201 (2014).

    2. Filonov, G.S., Moon, J.D., Svensen, N., et al. Broccoli: Rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution. J. Am. Chem. Soc. 136(46), 16299-16308 (2014).