Active • Host: E. coli • AA: 30-533 with a modified N-terminus and F-G loop • Tag: C-terminal His • MW: 59.1 kDa
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Thromboxane A Synthase (human, recombinant)

Item No. 33678

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CYP5A1
  • Cytochrome P450 5A1
  • TBXAS1
  • TXAS
  • TXA Synthase
Purity
≥90% estimated by SDS-PAGE
Source
Active recombinant C-terminal His-tagged thromboxane A synthase expressed in E.coli
Clone Designation
Slightly modified from wild-type
Amino Acids
30-533 with a modified N-terminus and F-G loop
MW
59.1 kDa
Formulation (Request formulation change)
50 mM Tris, pH 7.4, with 5% glycerol, 0.2 mM DTT, and 0.2 mM EDTA
UniProt Accession №
P24557
Shipping & Storage Information
Storage
-80°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 µg

    $637

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    Product Description

    Thromboxane A synthase (TXAS), also known as cytochrome P450 (CYP) isoform CYP5A1, is the enzyme that catalyzes the isomerization of prostaglandin H2 (PGH2; Item No. 17020) into thromboxane A2 (TXA2), a vasoconstrictor and an inducer of platelet aggregation.1,2 It also catalyzes the cleavage of PGH2 into malondialdehyde (MDA) and 12(S)-hydroxyheptadecatrienoic acid (12(S)-HHT; Item No. 34590), a leukotriene B4 (LTB4) receptor 2 (BLT2) agonist that also has a role in platelet aggregation.1 TXAS exists as a monomer and is composed of an N-terminal membrane anchor domain, a heme-binding catalytic residue, and several substrate-binding residues.3,4,5 It is expressed in numerous cells, including platelets, monocytes, and macrophages, as well as several tissues, and is localized to the endoplasmic reticulum.3 TXAS undergoes suicide inactivation during catalysis. Mice deficient in TXAS exhibit prolonged bleeding time and defective platelet aggregation.6 Cayman's Thromboxane A Synthase (human, recombinant) protein can be used for enzyme activity assay and Western blot (WB) applications. To construct this protein, the N-terminal amino acids 1-29 of human CYP5A1 were removed and replaced with a hydrophilic sequence. The F-G loop of TXAS was modified using sequences from rabbit CYP2C5 and CYP2C3. The C-terminal of this protein contains 6x-His tag followed by a stop codon.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Okuno, T., and Yokomizo, T. Biological functions of 12(S)-hydroxyheptadecatrienoic acid as a ligand of leukotriene B4 receptor 2. Inflamm. Regen. 38, 29 (2018).

    2. Hajeyah, A.A., Griffiths, W.J., Wang, Y., et al. The biosynthesis of enzymatically oxidized lipids. Front. Endocrinol. (Lausanne) 11, 591819 (2020).

    3. Ershov, P.V., Yablokov, E., Zgoda, V., et al. A new insight into subinteractomes of functional antagonists: Thromboxane (CYP5A1) and prostacyclin (CYP8A1) synthases. Cell Biol. Int. 45(6), 1175-1182 (2021).

    4. Ruan, K.H., Milfield, K., Kulmacz, R.J., et al. Comparison of the construction of a 3-D model for human thromboxane synthase using P450cam and BM-3 as templates: Implications for the substrate binding pocket. Protein Eng. 7(11), 1345-1351 (1994).

    5. Seo, M.-J., and Oh, D.-K. Prostaglandin synthases: Molecular characterization and involvement in prostaglandin biosynthesis. Prog. Lipid Res. 66, 50-68 (2017).

    6. Yu, I.-S., Lin, S.-R., Huang, C.-C., et al. TXAS-deleted mice exhibit normal thrombopoiesis, defective hemostasis, and resistance to arachidonate-induced death. Blood 104(1), 135-142 (2004).