Active • Host: E. coli • AA: 17-669 • Tag: N-terminal His • MW: 73 kDa
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GLS1 (human, recombinant)

Item No. 33737

Product Insert (PDF)
Technical Information
Synonyms
  • GAC
  • GAM
  • GLS
  • K-glutaminase
  • Glutaminase Kidney Isoform Mitochondrial
  • Glutaminase Transcript Variant 1
  • KGA
  • L-glutamine Amidohydrolase
Purity
≥80% estimated by SDS-PAGE
Source
Active recombinant human N-terminal His-tagged GLS1 expressed in E. coli
Amino Acids
17-699
MW
73 kDa
Formulation (Request formulation change)
40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 0.04% Tween 20, and 20% glycerol
UniProt Accession №
O94925
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    20 µg

    $642

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    Cayman Chemical
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    Product Description

    Glutaminase 1 (GLS1) is a mitochondrial enzyme that catalyzes the catabolism of glutamine to glutamate and ammonia.1,2 Alternative transcription of GLS, the gene encoding GLS1, produces two isozymes: kidney (K-type) glutaminase (KGA) and glutaminase C (GAC), which are expressed in kidney, brain, intestine, and fetal liver tissues.1 GLS expression is increased in multiple cancer types, including colorectal, prostate, and breast, and is associated with poor prognosis in ovarian, breast, gastric, and head and neck cancers.3 GLS1 is also essential to T helper 17 (Th17) cell differentiation, and pharmacologic inhibition of GLS1 ameliorates disease symptoms in mouse models of lupus, experimental autoimmune encephalomyelitis, and psoriasis.4,5 Cayman’s GLS1 (human, recombinant) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Curthoys, N.P., and Watford, M. Regulation of glutaminase activity and glutamine metabolism. Annu. Rev. Nutr. 15, 133-159 (1995).

    2. Matés, J.M., Segura, J.A., Martín-Rufián, M., et al. Glutaminase isoenzymes as key regulators in metabolic and oxidative stress against cancer. Curr. Mol. Med. 13(4), 514-534 (2013).

    3. Yu, W., Yang, X., Zhang, Q., et al. Targeting GLS1 to cancer therapy through glutamine metabolism. Clin. Transl. Oncol. 23(11), 2253-2268 (2021).

    4. Kono, M., Yoshida, N., Maeda, K., et al. Glutaminase 1 inhibition reduces glycolysis and ameliorates lupus-like disease in MRL/lpr mice and experimental autoimmune encephalomyelitis. Arthritis Rheumatol. 71(11), 1869-1878 (2019).

    5. Xia, X., Cao, G., Sun, G., et al. GLS1-mediated glutaminolysis unbridled by MALT1 protease promotes psoriasis pathogenesis. J. Clin. Invest. 130(10), 5180-5196 (2020).