A fluorogenic substrate for coronavirus Mpro
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Covidyte™ EN450

Item No. 33765

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Purity
≥95%
Emission
460 nm
Excitation
350 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: soluble
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 test

    $303

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    Product Description

    Viral main protease (Mpro), also known as 3C-like protease (3CLpro), is a viral protease that mediates the replication and transcription of severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2, the causative agents of SARS and COVID-19, respectively.1,2 Mpro digests SARS-CoV and SARS-CoV-2 viral polyproteins at 11 conserved sites, beginning with autolytic cleavage of itself, to release the functional peptides required for viral replication and transcription. Covidyte™ EN450 is a fluorogenic substrate for Mpro that contains 14 amino acids, KTSAVLQSGFRKME, which are recognized by Mpro. Upon enzymatic cleavage by Mpro, EDANS is separated from the Dabcyl quencher, displays excitation/emission maxima of 350/460 nm, respectively, and can be used to quantify Mpro activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kuo, C.-J., Chi, Y.-H., Hsu, J.T.-A., et al. Characterization of SARS main protease and inhibitor assay using a fluorogenic substrate. Biochem. Biophys. Res. Commun. 318(4), 862-867 (2004).

    2. Hung, H.-C., Ke, Y.-Y., Huang, S.Y., et al. Discovery of M protease inhibitors encoded by SARS-CoV-2. Antimicrob. Agents Chemother. 64(9), e00872-00820 (2020).