A fluorogenic substrate for coronavirus PLpro
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Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

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Product Categories

Product Type

Biochemicals

Covipyte™ EN450

Item No. 33769

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Purity
≥95%
Emission
460 nm
Excitation
350 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: soluble
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 test

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    Product Description

    Papain-like protease (PLpro) is a cysteine viral protease and component of the replicase complex in severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2, the causative agents of SARS and COVID-19, respectively.1,2 PLpro processes SARS-CoV and SARS-CoV-2 non-structural proteins 1-3, which, together with non-structural proteins 4-16, form the viral replicase complex, at three conserved sites, beginning with autolytic cleavage of itself.3,1,2 Covipyte™ EN450 is a fluorogenic substrate for PLpro that contains 9 amino acids, RELNGGAPI, which are recognized by PLpro. Upon enzymatic cleavage by PLpro, EDANS is separated from the Dabcyl quencher, displays excitation/emission maxima of 350/460 nm, respectively, and can be used to quantify PLpro activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Barretto, N., Jukneliene, D., Ratia, K., et al. The papain-like protease of severe acute respiratory syndrome coronavirus has deubiquitinating activity. J. Virol. 79(24), 15189-15198 (2005).

    2. Anirudhan, V., Lee, H., Cheng, H., et al. Targeting SARS-CoV-2 viral proteases as a therapeutic strategy to treat COVID-19. J. Med. Virol. (2021).

    3. Romano, M., Ruggiero, A., Squeglia, F., et al. A structural view of SARS-CoV-2 RNA replication machinery: RNA synthesis, proofreading and final capping. Cells 9(5), 1267 (2020).