For immunochemical detection of citrullinated myelin basic protein
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Citrullinated Myelin Basic Protein (R130) Monoclonal Antibody (Clone 3C6)

Item No. 33911

Product Insert (PDF)
Technical Information
Synonyms
  • citMBP R130
  • Citrullinated Myelin A1 Protein
  • Citrullinated Myelin Membrane Encephalitogenic Protein
Immunogen
Peptide from the C-terminal region of human myelin basic protein with citrulline at R130
Clone Designation
3C6
Formulation (Request formulation change)
25 µg of protein G affinity-purified monoclonal antibody
Storage Buffer
0.1 M Tris-glycine, pH 7.4, with 150 mM sodium chloride and 0.05% sodium azide
Host
Mouse
Isotype
IgG1
Applications
ELISA, IHC, WB
Cross Reactivity
(+) Citrullinated myelin basic protein (R130)
Species Reactivity
(+) Human(+) Mouse
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    25 µg

    $303

    Add
    Product Description

    Citrullinated myelin basic protein (citMBP) is a citrullinated form of MBP, a protein that is integral to myelin stability in the CNS.1 MBP contains 19 arginine residues, including the arginine residue at position 130 (R130) in human MBP, which corresponds to R128 in the mouse protein, that can be citrullinated by peptidylarginine deiminases (PADs), decreasing the positive charge of MBP.2,1,3 The loss of positive charge disrupts MBP-lipid interactions, leading to myelin destabilization and loss and increases its susceptibility for degradation. MBP is degraded by the myelin-associated protease cathepsin D, which leads to exposure and release of immunodominant epitopes.1,4 The percentage of MBP that is citrullinated is increased in the postmortem brain from patients with multiple sclerosis (MS), with an even greater percentage citrullinated in Marburg disease, a more severe form of MS.1 citMBP isolated from patients with multiple sclerosis (MS) is degraded by cathepsin D at a higher rate than MBP isolated from healthy controls.1,4 Cayman's Citrullinated Myelin Basic Protein (R130) Monoclonal Antibody (Clone 3C6) can be used for ELISA, immunohistochemistry (IHC), and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yang, L., Tan, D., and Piao, H. Myelin basic protein citrullination in multiple sclerosis: A potential therapeutic target for the pathology. Neurochem. Res. 41(8), 1845-1856 (2016).

    2. Jang, B., Jeon, Y.-C., Shin, H.-Y., et al. Myelin basic protein citrullination, a hallmark of central nervous system demyelination, assessed by novel monoclonal antibodies in prion diseases. Mol. Neurobiol. 55(4), 3172-3184 (2018).

    3. Mastronardi, F.G., and Moscarello, M.A. Molecules affecting myelin stability: A novel hypothesis regarding the pathogenesis of multiple sclerosis. J. Neurosci. Res. 80(3), 301-308 (2005).

    4. Cao, L., Goodin, R., Wood, D., et al. Rapid release and unusual stability of immunodominant peptide 45-89 from citrullinated myelin basic protein. Biochemistry 38(19), 6157-6163 (1999).