A fluorescent membrane potential indicator
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

DiBAC4(3)

Item No. 33924

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
1,3-dibutyl-5-[3-(1,3-dibutylhexahydro-2,4,6-trioxo-5-pyrimidinyl)-2-propen-1-ylidene]-2,4,6(1H,3H,5H)-pyrimidinetrione
CAS Number
70363-83-6
Synonyms
  • Bis(1,3-Dibutylbarbituric Acid) Trimethine Oxonol
Molecular Formula
C27H40N4O6
Formula Weight
516.6
Purity
≥95%
Emission
516 nm
Excitation
490 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMSO: 15 mg/mlDMSO:PBS (pH 7.2) (1:2): 0.33 mg/mlEthanol: 5 mg/ml
λmax
495 nm
SMILES
O=C1/C(C(N(CCCC)C(N1CCCC)=O)=O)=C/C=CC2C(N(CCCC)C(N(CCCC)C2=O)=O)=O
InChi Code
InChI=1S/C27H40N4O6/c1-5-9-16-28-22(32)20(23(33)29(26(28)36)17-10-6-2)14-13-15-21-24(34)30(18-11-7-3)27(37)31(25(21)35)19-12-8-4/h13-15,20H,5-12,16-19H2,1-4H3
InChi Key
ATJCYSYHWGQAET-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 mg

    $62

    Add

    10 mg

    $107

    Add

    25 mg

    $222

    Add

    50 mg

    $383

    Add
    Product Description

    DiBAC4(3) is a negatively charged fluorescent membrane potential indicator.1 Upon membrane depolarization, DiBAC4(3) enters the cytosol, binds to lipid membranes and intracellular proteins, and displays excitation/emission maxima of 490/516 nm, respectively.1,2 It has been used to measure the membrane potential of live or fixed mammalian and bacterial cells by flow cytometry or fluorescence microscopy.2,3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yamada, A., Gaja, N., Ohya, S., et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn. J. Pharmacol. 86(3), 342-350 (2001).

    2. Warren, E.A.K., and Payne, C.K. Cellular binding of nanoparticles disrupts the membrane potential. RSC Adv. 5(18), 13660-13666 (2015).

    3. Klapperstück, T., Glanz, D., Klapperstück, M., et al. Methodological aspects of measuring absolute values of membrane potential in human cells by flow cytometry. Cytometry A. 75(7), 593-608 (2009).

    4. Mason, D.J., Allman, R., Stark, J.M., et al. Rapid estimation of bacterial antibiotic susceptibility with flow cytometry. J. Microsc. 176(Pt 1), 8-16 (1994).