A colorimetric serine protease substrate
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Nα-Benzoyl-DL-Arginine-pNA (hydrochloride)

Item No. 33934

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[4-[(aminoiminomethyl)amino]-1-[[(4-nitrophenyl)amino]carbonyl]butyl]-benzamide, monohydrochloride
CAS Number
911-77-3
Synonyms
  • Nα-Benzoyl-DL-Arginine-p-nitroanilide
  • DL-BAPNA
  • NSC 83264
Molecular Formula
C19H22N6O4 • HCl
Formula Weight
434.9
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 10 mg/mlDMSO:PBS (pH 7.2) (1:8): 0.11 mg/ml
λmax
225, 312 nm
SMILES
O=C(C1=CC=CC=C1)NC(CCCNC(N)=N)C(NC2=CC=C([N+]([O-])=O)C=C2)=O.Cl
InChi Code
InChI=1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H
InChi Key
DEOKFPFLXFNAON-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

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    10 g

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    Product Description

    Nα-Benzoyl-DL-arginine-pNA (DL-BAPNA) is a colorimetric substrate for serine proteases, including β-secretase 1 (BACE1), calpain, and papain.1,2 Serine proteases bind to and cleave DL-BAPNA to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of serine protease activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Mancini, F., Naldi, M., Cavrini, V., et al. Multiwell fluorometric and colorimetric microassays for the evaluation of beta-secretase (BACE-1) inhibitors. Anal. Bioanal. Chem. 388(5-6), 1175-1183 (2007).

    2. Chung, M.-C., Lee, H.-J., Chun, H.K., et al. Penicillide, a nonpeptide calpain inhibitor, produced by Penicillium sp. F60760. J. Microbiol. Biotechnol. 8(2), 188-190 (1998).