A chromogenic and electrochemical substrate for alkaline phosphatase
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3-Indoxyl Phosphate (sodium salt)

Item No. 33936

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3-(dihydrogen phosphate), 1H-Indol-3-ol, disodium salt
CAS Number
3318-43-2
Synonyms
  • 3-IP
Molecular Formula
C8H6NO4P • 2Na
Formula Weight
257.1
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
PBS (pH 7.2): 10 mg/ml
λmax
222 nm
SMILES
O=P([O-])(OC1=CNC2=C1C=CC=C2)[O-].[Na+].[Na+]
InChi Code
InChI=1S/C8H8NO4P.2Na/c10-14(11,12)13-8-5-9-7-4-2-1-3-6(7)8;;/h1-5,9H,(H2,10,11,12);;/q;2*+1/p-2
InChi Key
WCSKWBKPKXJWEG-UHFFFAOYSA-L
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    250 mg

    $121

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    1 g

    $334

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    Product Description

    3-Indoxyl phosphate is a chromogenic and electrochemical substrate for alkaline phosphatase.1,2 Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity.1 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Tsou, K.C., and Su, H.C.F. A new colorimetric method for the determination of alkaline phosphatase with indoxyl phosphate. Anal. Biochem. 11(1), 54-64 (1965).

    2. Fanjul-Bolado, P., González-García, M.B., and Costa-García, A. Detection of leucoindigo in alkaline phosphatase and peroxidase based assays using 3-indoxyl phosphate as substrate. Anal. Chim. Acta 534(2), 231-238 (2005).