Host: Calf thymus • AA: 1-213 (H1.2), 1-221 (H1.3), and 1-219 (H1.4) • MW: 21.3 (H1.2), 22.1 (H1.3), and 21.9 (H1.4) kDa
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Histone H1 (bovine)

Item No. 33943

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Purity
≥70% estimated by SDS-PAGE
Source
Calf thymus
Amino Acids
1-213 (H1.2), 1-221 (H1.3), and 1-219 (H1.4)
MW
21.3 (H1.2), 22.1 (H1.3), and 21.9 (H1.4) kDa
Formulation (Request formulation change)
A solution in water (frozen)
UniProt Accession №
P02253, A7MAZ5, G3MWV5
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    25 mg

    $247

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    Cayman Chemical
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    Product Description

    Histone H1 is a nuclear protein that interacts with linker DNA regions between nucleosomes to provide nucleosome core structure stabilization.1,2 Histone H1 subtypes are comprised of a short N-terminal domain, a central globular domain necessary for DNA interaction, and long C-terminal domain that determines subtype binding properties by its variability.2 Histones H1.1-H1.5 are expressed in somatic cells in a replication-dependent manner, and cell-type specificity varies based on histone H1 subtype.3 Histone H1 is involved in chromatin organization, gene expression, protection of DNA from digestion by micrococcal nuclease, the DNA damage response, and cell differentiation.2,4 Knockdown of H1-2 and H1-4 decreases proliferation of T47D breast cancer cells, and H1-2 knockdown also induces cell cycle arrest at the G1 phase in the same cells.5 Histone H1 is highly post-translationally modified, including phosphorylation, acetylation, citrullination, ubiquitination, and methylation.4 Phosphorylation of histone H1 at Thr146 is associated with increasing breast cancer tumor grade.6 Cayman’s Histone H1 (bovine) protein can be used as a substrate for enzyme activity assays, as well as for Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Allan, J., Hartman, P.G., Crane-Robinson, C., et al. The structure of histone H1 and its location in chromatin. Nature 288(5792), 675-679 (1980).

    2. Roque, A., Ponte, I., and Suau, P. Interplay between histone H1 structure and function. Biochim. Biophys. Acta 1859(3), 444-454 (2016).

    3. Millán-Ariño, L., Izquierdo-Bouldstridge, A., and Jordan, A. Specificities and genomic distribution of somatic mammalian histone H1 subtypes. Biochim. Biophys. Acta 1859(3), 510-519 (2015).

    4. Andrés, M., García-Gomis, D., Ponte, I., et al. Histone H1 post-translational modifications: Update and future perspectives. Int. J. Mol. Sci. 21(16), 5941 (2020).

    5. Sancho, M., Diani, E., Beato, M., et al. Depletion of human histone H1 variants uncovers specific roles in gene expression and cell growth. PLoS Genet. 4(10), e1000227 (2008).

    6. Harshman, S.W., Hoover, M.E., Huang, C., et al. Histone H1 phosphorylation in breast cancer. J. Proteome Res. 13(5), 2453-2467 (2014).