Interleukin-16 Monoclonal Antibody (Clone 55)

IL-16 is a cytokine that has roles as a T cell chemoattractant and modulator of T cell activation.^1,2 It is synthesized as a 631-amino acid precursor protein, precursor IL-16, that is cleaved by caspase-3 at serine 511 (Ser⁵¹¹), which produces the N-terminal peptide, pro-IL-16, and the 121-amino acid C-terminal peptide, mature IL-6, both of which are biologically active.² Pro-IL-16 translocates to the nucleus, where it acts as a transcriptional repressor of S-phase kinase-associated protein 2 (Skp2), preventing cell cycle entry, whereas mature IL-16 is secreted upon antigen or pro-inflammatory cytokine stimulation and acts as a CD4+ T cell chemoattractant.^2,3 Precursor IL-16 is constitutively expressed by CD8⁺ or CD4⁺ T cells.³ Caspase-3-mediated cleavage of precursor IL-16 is constitutive in CD8⁺ T cells, which store mature IL-16 in vesicles until antigen, mitogen, or vasoactive amine stimulation, whereas caspase-3 activation by mitogens or antigens is a prerequisite for precursor IL-16 processing in CD4⁺ T cells. IL-16 production can also be induced by growth factor, cytokine, or mitogen stimulation in other leukocytes, including macrophages, dendritic cells, mast cells, and eosinophils. IL-16 has roles in numerous inflammatory conditions.⁴ Serum IL-16 levels are increased in patients infected with severe acute respiratory syndrome coronavirus (SARS-CoV) or hepatitis C virus (HCV), and IL-16 levels are increased in the CNS or synovial fluid of patients with multiple sclerosis or rheumatoid arthritis, respectively. Cayman's Interleukin-16 Monoclonal Antibody (Clone 55) can be used for immunohistochemistry (IHC) applications. The antibody recognizes IL-16 from human samples.

WARNING This product is not for human or veterinary use.

1. Mathy, N.L., Scheuer, W., Lanzendörfer, M., et al. Interleukin-16 stimulates the expression and production of pro-inflammatory cytokines by human monocytes. Immunology 100(1), 63-69 (2000).

2. Richmond, J., Tuzova, M., Cruikshank, W., et al. Regulation of cellular processes by interleukin-16 in homeostasis and cancer. J. Cell. Physiol. 229(2), 139-147 (2014).

3. Cruikshank, W.W., Kornfeld, H., and Center, D.M. Interleukin-16. J. Leukoc. Biol. 67(6), 757-766 (2000).

4. Glass, W.G., Sarisky, R.T., and Del Vecchio, A.M. Not-so-sweet sixteen: The role of IL-16 in infectious and immune-mediated inflammatory diseases. J. Interferon. Cytokine Res. 26(8), 511-520 (2006).