A chelating agent
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EGTA

Item No. 33963

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,12-bis(carboxymethyl)-6,9-dioxa-3,12-diazatetradecanedioic acid
CAS Number
67-42-5
Synonyms
  • Egtazic Acid
  • Ethylene Glycol bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic Acid
  • Ethylene Glycol Tetraacetic Acid
  • GEDTA
  • NSC 615010
Molecular Formula
C14H24N2O10
Formula Weight
380.4
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
1 M NaOH: SolublePBS (pH 7.2): Slightly soluble
SMILES
O=C(CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O)O
InChi Code
InChI=1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
InChi Key
DEFVIWRASFVYLL-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    25 g

    $66

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    50 g

    $103

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    100 g

    $141

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    250 g

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    Product Description

    EGTA is an aminopolycarboxylic acid chelating agent.1 It is selective for calcium over magnesium with Kapp values for calcium ranging from 6.93 to 10.7 x 105 M-1 in various buffers at pH 6.8 and 20°C. EGTA (5 mM) inhibits apoptosis induced by the sarcoplasmic/endoplasmic Ca2+-ATPase (SERCA) inhibitor thapsigargin (Item No. 10522) in isolated rat thymocytes.2 It has been used as an irrigation agent to remove the smear layer from the pulp canal wall on extracted human teeth.3 Intranasal administration of EGTA enhances adenovirus-mediated gene transfer of LacZ to the trachea and main bronchi in mice by 8-fold.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Harafuji, H., and Ogawa, Y. Re-examination of the apparent binding constant of ethylene glycol bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid with calcium around neutral pH. J. Biochem. 87(5), 1305-1312 (1980).

    2. Jiang, S., Chow, S.C., Nicotera, P., et al. Intracellular Ca2+ signals activate apoptosis in thymocytes: Studies using the Ca2+-ATPase inhibitor thapsigargin. Exp. Cell. Res. 212(1), 84-92 (1994).

    3. Viswanath, D., Hedge, A.M., and Munshi, A.K. The removal of the smear layer using EGTA: A scanning electron microscopic study. J. Clin. Pediatr. Dent. 28(1), 69-74 (2003).

    4. Gregory, L.G., Harbottle, R.P., Lawrence, L., et al. Enhancement of adenovirus-mediated gene transfer to the airways by DEAE dextran and sodium caprate in vivo. Mol. Ther. 7(1), 19-26 (2003).