Active • Host: Insect cells • AA: 1-469 (full length) • Tag: C-terminal His • MW: 55 kDa
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GBA3 (human, recombinant)

Item No. 33991

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • cBGL1
  • Cytosolic β-Glucosidase
  • Cytosolic β-Glucosidase-like Protein-1
  • Klotho-related Protein
  • KLrP
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Active recombinant human C-terminal His-tagged GBA3 expressed in insect cells
Amino Acids
1-469 (full length)
MW
55 kDa
Formulation (Request formulation change)
Lyophilized from sterile 20 mM Tris, 300 mM sodium chloride, pH 7.5, with 10% glycerol, 5% trehalose, 5% mannitol, and 0.01% tween-80
Host
Insect cells
UniProt Accession №
Q9H227
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $361

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    Product Description

    Cytosolic β-glucosidase (GBA3) is a neutral β-glucocerebrosidase.1,2 It is composed of a single glycoside hydrolase domain and hydrolyzes glucosyl- and galactosylceramides. GBA3 is expressed in the liver, small intestine, colon, spleen, and kidneys.3 In vitro, knockdown of GBA3 inhibits mitotic arrest and promotes resistance to paclitaxel (Item No. 10461) in colon cancer cells.4 Tumor levels of GBA3 are lower in patients with hepatocellular carcinoma and decreased expression is associated with poor prognosis.5 Unlike GBA1, polymorphisms in GBA3 are not associated with Gaucher disease, a lysosomal storage disorder.6 Cayman’s GBA3 (human, recombinant) protein can be used for enzyme activity assays. This protein consists of 480 amino acids and has a calculated molecular weight of 55 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hayashi, Y., and Ito, M. Klotho-related protein KLrP: Structure and functions. Vitamins and Hormones 101, 1-16 (2016).

    2. Astudillo, L., Therville, N., Colacios, C., et al. Glucosylceramidases and malignancies in mammals. Biochimie 125, 267-280 (2016).

    3. Yahata, K., Mori, K., Arai, H., et al. Molecular cloning and expression of a novel klotho-related protein. J. Mol. Med. (Berl.) 78(7), 389-394 (2000).

    4. Swanton, C., Marani, M., Pardo, O., et al. Regulators of mitotic arrest and ceramide metabolism are determinants of sensitivity to paclitaxel and other chemotherapeutic drugs. Cancer Cell 11(6), 498-512 (2007).

    5. Ying, J.F., Zhang, Y.N., Song, S.S., et al. Decreased expression of GBA3 correlates with a poor prognosis in hepatocellular carcinoma patients. Neoplasma 67(5), 1139-1145 (2020).

    6. Beutler, E., Beutler, L., and West, C. Mutations in the gene encoding cytosolic β-glucosidase in Gaucher disease. J. Lab. Clin. Med. 144(2), 65-68 (2004).