An amine-reactive fluorescent protein-labeling agent
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5-(4,6-Dichloro-s-triazin-2-ylamino)fluorescein (hydrochloride)

Item No. 34080

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-[(4,6-dichloro-1,3,5-triazin-2-yl)amino]-3′,6′-dihydroxy-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, monohydrochloride
CAS Number
21811-74-5
Synonyms
  • Dichlorotriazinylaminofluorescein
  • DTAF
Molecular Formula
C23H12Cl2N4O5 • HCl
Formula Weight
531.7
Purity
≥98%
Emission
513 nm
Excitation
492 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 1 mg/mlEthanol: 1 mg/mlPBS (pH 7.2): insol
λmax
211, 225, 277 nm
SMILES
OC1=CC=C2C3(C4=CC=C(NC5=NC(Cl)=NC(Cl)=N5)C=C4C(O3)=O)C6=CC=C(O)C=C6OC2=C1.Cl
InChi Code
InChI=1S/C23H12Cl2N4O5.ClH/c24-20-27-21(25)29-22(28-20)26-10-1-4-14-13(7-10)19(32)34-23(14)15-5-2-11(30)8-17(15)33-18-9-12(31)3-6-16(18)23;/h1-9,30-31H,(H,26,27,28,29);1H
InChi Key
TZGKHPXUAULWOC-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 mg

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    100 mg

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    Product Description

    5-(4,6-Dichloro-s-triazin-2-ylamino)fluorescein (DTAF) is an amine-reactive fluorescent probe for labeling proteins.1 It displays excitation/emission maxima of 492/513 nm, respectively. DTAF has been conjugated to IgG for use as fluorescent antibodies and as a pre-column or on-column reagent to label amino acids and amino acid-containing compounds.1,2,3 It has also been used as a derivatizing agent in the analysis of ephedrine and pseudoephedrine from various sources.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bakeslee, D., and Baines, M.G. Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG. J. Immunol. Methods 13(3-4), 305-320 (1976).

    2. De Boer, S.H., and Wieczorek, A. Production of monoclonal antibodies to Corynebacterium spedonicum. Phytopathology 74(12), 1431-1434 (1984).

    3. Wang, W., Li, C., Li, Y., et al. Rapid and ultrasensitive determination of ephedrine and pseudoephedrine derivatizated with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein by micellar electrokinetic chromatography with laser-induced fluorescence detection. J. Chromatogr. A. 1102(1-2), 273-279 (2005).